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For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.

HPLC Method for Separation of Hydrophobic, Cationic and Anionic Surfactants on Newcrom BH by SIELC Technologies

Surfactants, also known as surface-active agents, are compounds that lower the surface tension (or interfacial tension) between two liquids or between a liquid and a solid. Surfactants may act as detergents, wetting agents, emulsifiers, foaming agents, or dispersants.

They are often classified according to the charge of the polar head group:

Anionic Surfactants: These surfactants have a negative charge on their polar head group. Common examples include soap, sodium laureth sulfate, and sodium lauryl sulfate. They are commonly used in detergents and shampoos due to their ability to emulsify oils and hold dirt in suspension, so it can be rinsed away.

Cationic Surfactants: These surfactants have a positive charge on their polar head group. Examples include cetyltrimethylammonium bromide (CTAB) and benzalkonium chloride. These are often used as antiseptics and can also be found in hair conditioners because they reduce static cling.

Nonionic Surfactants: These surfactants have no charge on their polar head group. Examples include alcohol ethoxylates, nonylphenol ethoxylates, and polysorbates. Nonionic surfactants are often used in laundry and dishwasher detergents.

Sodium, which has a chemical symbol of Na, is a soft alkali metal that is highly reactive.  It is found in abundance in everyday materials like table salt, sea water, and even the Earth’s crust. It is crucial  for the body’s function and fluid balance.

1-Ethylpyridinium bromide is an ionic liquid with the chemical formula C₇H₁₀BrN. It is primarily used as a solvent at temperatures below 100^oC catalysts, and electrolytes in , It has an ethyl group attached to a pyridinium ring, and its counterion is bromide (Br-). You can find detailed UV spectra of 1-Ethylpyridinium bromide and information about its various lambda maxima by visiting the following link.

Benzalkonium chloride, also known as Zephiran or Alkyldimethylbenzylammonium chloride, is a positively charged surfactant with the chemical formula C₆H₅CH₂N(CH₃)₂RCl. It is often used as a pharmaceutical preservative and as an antiseptic. You can find detailed UV spectra of Benzalkonium chloride and information about its various lambda maxima by visiting the following link.

Cetylpyridinium Chloride, also written as CPC, is a cationic quaternary ammonium compound with the chemical formula C₂₁H₃₈ClN. It is commonly used in mouthwashes, toothpastes, lozenges, throat sprays, breath sprays, and nasal sprays due to it’s antiseptic abilities. You can find detailed UV spectra of Cetylpyridinium Chloride and information about its various lambda maxima by visiting the following link.

Chloride is typically used to refer to a compound or molecule that contains a chlorine anion: Cl^–. It is an electrolyte that is essential for bodily functions including blood pH, fluid balance, cellular functions, and more. Imbalance of chloride can indicate underlying health problems.

1-Pentanesulfonic acid is an organosulfonic acid with the chemical formula C₅H₁₂O₃S. It is primarily used in it’s sodium salt form as an anionic pairing agent. You can find detailed UV spectra of 1-Pentanesulfonic acid and information about its various lambda maxima by visiting the following link.

Lauric acid, also known as dodecanoic acid, is a saturated fatty acid. Its chemical formula is CH₃(CH₂)₁₀COOH. It is white in color, and it is a solid at room temperature with a melting point of around 44 degrees Celsius (111 degrees Fahrenheit). It is relatively inexpensive and has a long shelf life, which makes it an attractive ingredient in various commercial products. Lauric acid is found naturally in various plant and animal fats and oils, but the largest amounts of lauric acid are found in coconut oil and palm kernel oil. In these oils, lauric acid can make up to 50% of the total fat content. It is commonly used in the manufacturing of soaps and cosmetics due to its ability to form a lather when mixed with water. It is also used in food for its role in digestion and metabolic processes. It’s a medium-chain fatty acid, which means it is more easily absorbed and utilized by the body compared to long-chain fatty acids. You can find detailed UV spectra of Dodecanoic acid (Lauric acid) and information about its various lambda maxima by visiting the following link.

p-Toluenesulfonic Acid, also known as tosylic acid, which are written as PTSA, pTSA, pTsOH, and TsOH, is an organic compound with the chemical formula C₇H₈O₃S. It is used primarily in organic synthesis. You can find detailed UV spectra of p-Toluenesulfonic Acid and information about its various lambda maxima by visiting the following link.

1-Octanesulfonic acid is an anionic organosulfonic acid with the chemical formula C₈H₁₈O₃S. It is primarily used in it’s sodium salt form as an ion-pairing agent. You can find detailed UV spectra of 1-Heptanesulfonic acid and information about its various lambda maxima by visiting the following link.

Sodium dodecyl sulfate (SDS), or sodium lauryl sulfate (SLS), is a surfactant used in cleaning, hygienic, and food products. It has a chemical formula C₁₂H₂₅NaSO₄, meaning it has twelve carbon hydrophobic tail attached to a polar sulfate. At low concentrations, it is used as a whipping aid and emulsifier in foods containing egg whites. It has a similar use in pharmaceutics as an ionic solubilizer and, also, an emulsifier. In laboratory uses, it is used as a component for lysing cells during RNA extraction or DNA extraction. You can find detailed UV spectra of Sodium dodecyl sulfate and information about its various lambda maxima by visiting the following link.

Benzalkonium chloride, Cetylpyridinium Chloride, 1-Pentanesulfonic acid, Dodecanoic acid (Lauric acid), p-Toluenesulfonic Acid (PTSA), 1-Octanesulfonic acid, Sodium dodecyl sulfate, 1-Ethylpyridinium bromide can be retained, separated, and analyzed using a reverse-phase [colimn] column. The mobile phase for this method consists of water, acetonitrile (MeCN), and Ammonium formate, which serves as a buffer. This analytical method can be detected with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).

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Description

HPLC Method for Separation of Hydrophobic, Cationic, Nonionic and Anionic Surfactants on Newcrom BH by SIELC Technologies

Surfactants, also known as surface-active agents, are compounds that lower the surface tension (or interfacial tension) between two liquids or between a liquid and a solid. Surfactants may act as detergents, wetting agents, emulsifiers, foaming agents, or dispersants.

They are often classified according to the charge of the polar head group:

Anionic Surfactants: These surfactants have a negative charge on their polar head group. Common examples include soap, sodium laureth sulfate, and sodium lauryl sulfate. They are commonly used in detergents and shampoos due to their ability to emulsify oils and hold dirt in suspension, so it can be rinsed away.

Cationic Surfactants: These surfactants have a positive charge on their polar head group. Examples include cetyltrimethylammonium bromide (CTAB) and benzalkonium chloride. These are often used as antiseptics and can also be found in hair conditioners because they reduce static cling.

Nonionic Surfactants: These surfactants have no charge on their polar head group. Examples include alcohol ethoxylates, nonylphenol ethoxylates, and polysorbates. Nonionic surfactants are often used in laundry and dishwasher detergents.

Sodium, which  has a chemical symbol of Na, is a soft alkali metal that is highly reactive.  It is found in abundance in everyday materials like table salt, sea water, and even the Earth’s crust. It is crucial  for the body’s function and fluid balance.

Benzalkonium chloride, also known as Zephiran or Alkyldimethylbenzylammonium chloride, is a positively charged surfactant with the chemical formula C₆H₅CH₂N(CH₃)₂RCl. It is often used as a pharmaceutical preservative and as an antiseptic. You can find detailed UV spectra of Benzalkonium chloride and information about its various lambda maxima by visiting the following link.

Cetylpyridinium Chloride, also written as CPC, is a cationic quaternary ammonium compound with the chemical formula C₂₁H₃₈ClN. It is commonly used in mouthwashes, toothpastes, lozenges, throat sprays, breath sprays, and nasal sprays due to it’s antiseptic abilities. You can find detailed UV spectra of Cetylpyridinium Chloride and information about its various lambda maxima by visiting the following link.

Triton X100 is a nonionic surfactant with the chemical formula C₁₄H₂₂O(C₂H₄O)_n. It is primarily used as a detergent in laboratories, but it has a wide variety of laboratory uses. Commercially, it can be found in cleaning compounds. You can find detailed UV spectra of Triton X100 and information about its various lambda maxima by visiting the following link.

Chloride is typically used to refer to a compound or molecule that contains a chlorine anion: Cl^–. It is an electrolyte that is essential for bodily functions including blood pH, fluid balance, cellular functions, and more. Imbalance of chloride can indicate underlying health problems.

1-Pentanesulfonic acid is an organosulfonic acid with the chemical formula C₅H₁₂O₃S. It is primarily used in it’s sodium salt form as an anionic pairing agent. You can find detailed UV spectra of 1-Pentanesulfonic acid and information about its various lambda maxima by visiting the following link.

1-Hexanesulfonic acid is an alkyl sulfonate compound with the chemical formula C₆H₁₃O₃S^– (or C₆H₁₃NaO₃S as a sodium salt). It is primarily used in it’s sodium salt form as an ionic pairing agent in high-performance liquid chromatography.

1-Heptanesulfonic acid is an organosulfonic acid with the chemical formula C₇H₁₆O₃S. It is primarily used in it’s sodium salt form as an ion-pairing agent. You can find detailed UV spectra of 1-Heptanesulfonic acid and information about its various lambda maxima by visiting the following link.

1-Octanesulfonic acid is an anionic organosulfonic acid with the chemical formula C₈H₁₈O₃S. It is primarily used in it’s sodium salt form as an ion-pairing agent. You can find detailed UV spectra of 1-Heptanesulfonic acid and information about its various lambda maxima by visiting the following link.

1-Decanesulfonic acid is an alkyl sulfonic acid with the chemical formula C₁₀H₂₁NaO₃S. It is primarily used in it’s sodium salt form as an ion-pairing agent. You can find detailed UV spectra of 1-Decanesulfonic acid and information about its various lambda maxima by visiting the following link.

Sodium dodecyl sulfate (SDS), or sodium lauryl sulfate (SLS), is a surfactant used in cleaning, hygienic, and food products. It has a chemical formula C₁₂H₂₅NaSO₄, meaning it has twelve carbon hydrophobic tail attached to a polar sulfate. At low concentrations, it is used as a whipping aid and emulsifier in foods containing egg whites. It has a similar use in pharmaceutics as an ionic solubilizer and, also, an emulsifier. In laboratory uses, it is used as a component for lysing cells during RNA extraction or DNA extraction. You can find detailed UV spectra of Sodium dodecyl sulfate and information about its various lambda maxima by visiting the following link.

Benzalkonium chloride, Cetylpyridinium Chloride, Triton X100, 1-Pentanesulfonic acid, 1-Hexanesulfonic acid, sodium salt, 1-Heptanesulfonic acid, 1-Decanesulfonic acid, Sodium dodecyl sulfate, 1-Octanesulfonic acid can be retained, separated, and analyzed using a reverse-phase Newcrom BH, 4.6 x 250 mm, 5 µm, 100 A, dual ended column. The mobile phase for this method consists of water, acetonitrile (MeCN), and Ammonium formate, which serves as a buffer. This analytical method can be detected with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).

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Description

HPLC Method for Cetylpyridinium Chloride, Chlorhexidine on Primesep B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Cetylpyridinium Chloride, Chlorhexidine.

Chlorhexidine gluconate, or simply chlorhexidine, is a biguanide used as an antiseptic and disinfectant. It is a component of mouthwash rinses that has been shown to reduce plaque, gingivitis and oral bacteria. It’s also used as a topical agent for skin disinfection. Cetylpyridinium chloride is another type of antiseptic used in mouthwash rinses. Both compounds are cationic.

You can find detailed UV spectra of Chlorhexidine and information about its various lambda maxima by visiting the following link.

You can find detailed UV spectra of Cetylpyridinium Chloride and information about its various lambda maxima by visiting the following link.

Cetylpyridinium Chloride, Chlorhexidine can be separated using HPLC on SIELC’s reverse-phase (RP) mixed-mode Primesep B column with the mobile phase of acetonitrile (ACN) and water with formic acid buffer and UV detected at 270nm.

Cetylpyridinium chloride is hydrophobic basic compound and triethylene glycol is hydrophilic neutral compounds. Quantitative analysis of both compounds is problematic due to a different nature of these two analytes. Both compounds were analyzed on an Obelisc N column in HILIC/cation-exchange mode. Cetylpyridinium chloride is retained by cation-exchange mechanism, and triethylene glycol is retained by HILIC mechanism. Mixed-mode HILIC approach allows to retain compounds either based on multiple or single mechanisms interaction, thus providing a valuable approach for analysis. Cetylpyridinium chloride and triethylene glycol can be monitored by combination of UV and ELSD/CAD.

Cetylpyridinium Chloride is an antiseptic agent used in mouthwash or dental care for oral and throat care. Primesep D retains cetylpyridinium by a combination of reversed-phase and ion-exchange mechanisms. The anion-exchange properties of the column can be adjusted by the TFA concentration in the mobile phase and the reversed-phase properties are adjusted by the percentage of acetontrile. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) and UV detection at 250 nm.

Surfactants are molecules that contain both hydrophilic and hydrophobic groups, usually in the form of a hydrophilic head and a hydrophobic tail. Surfactants are used in detergents where they can form micelles around hydrophobic dirt molecules and wash them away. Triton X-100 is a surfactant with a hydrophilic polyethylene oxide chain that can be separated on a Primesep D reverse-phase HPLC column based on the number of oxide units in the chain. The mobile phase is water, acetonitrile (MeCN, ACN) and sulfuric acid as buffer. UV detection at 210nm.

Primesep B separates tertiary amines, such as cetylpyridinium with symmetrical peak shape by a combination of reversed-phase and ion-exclusion mechanisms. The embedded basic functional group on the stationary phase shields the underlying silanols to prevent peak tailing. Retention time can be changed by changing either organic content or acid content in the mobile phase. C18 reversed-phase columns do not typically show this tuning ability with acid content. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 250 nm.