Cetylpyridinium Methods with Good Efficiency and Peak Symmetry

Primesep B separates tertiary amines, such as cetylpyridinium with symmetrical peak shape by a combination of reversed-phase and ion-exclusion mechanisms. The embedded basic functional group on the stationary phase shields the underlying silanols to prevent peak tailing. Retention time can be changed by changing either organic content or acid content in the mobile phase. C18 reversed-phase columns do not typically show this tuning ability with acid content. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 250 nm.


Column Primesep B,  4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA
Flow Rate 1.0 ml/min
Detection UV 210nm


Class of Compounds Surfactant,  Hydrophobic, Ionizable
Analyzing Compounds Cetylpyridinium Chloride

Application Analytes:

Cetylpyridinium Chloride
Pyridinium Ion
Quaternary Amines