HPLC Method for Analysis of Cetylpyridinium Chloride on Primesep D Column

Cetylpyridinium Chloride is an antiseptic agent used in mouthwash or dental care for oral and throat care. Primesep D retains cetylpyridinium by a combination of reversed-phase and ion-exchange mechanisms. The anion-exchange properties of the column can be adjusted by the TFA concentration in the mobile phase and the reversed-phase properties are adjusted by the percentage of acetontrile. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) and UV detection at 250 nm.

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Cetylpyridinium Chloride
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.