HPLC Method for Cetylpyridinium Chloride on Primesep D by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Cetylpyridinium Chloride.
Cetylpyridinium Chloride, also written as CPC, is a cationic quaternary ammonium compound with the chemical formula C21H38ClN. It is commonly used in mouthwashes, toothpastes, lozenges, throat sprays, breath sprays, and nasal sprays due to it’s antiseptic abilities. You can find detailed UV spectra of Cetylpyridinium Chloride and information about its various lambda maxima by visiting the following link
Primesep D retains Cetylpyridinium Chloride by a combination of reversed-phase and ion-exchange mechanisms. The anion-exchange properties of the column can be adjusted by the TFA concentration in the mobile phase and the reversed-phase properties are adjusted by the percentage of acetontrile. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) and UV detection at 250 nm.
Condition
| Column | Primesep D, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O |
| Buffer | TFA |
| Flow Rate | 1.0 ml/min |
| Detection | UV |
Description
| Class of Compounds | Surfactant |
| Analyzing Compounds | Cetylpyridinium Chloride |
Application Column
Primesep D
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
