Surfactants are molecules that contain both hydrophilic and hydrophobic groups, usually in the form of a hydrophilic head and a hydrophobic tail. Surfactants are used in detergents where they can form micelles around hydrophobic dirt molecules and wash them away. Triton X-100 is a surfactant with a hydrophilic polyethylene oxide chain that can be separated on a Primesep D reverse-phase HPLC column based on the number of oxide units in the chain. The mobile phase is water, acetonitrile (MeCN, ACN) and sulfuric acid as buffer. UV detection at 210nm.
| Column | Primesep D, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H2SO4- 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210nm |
| Class of Compounds |
Surfactant, Hydrophobic, Ionizable |
| Analyzing Compounds | Triton X-100, Cetylpyridinium Chloride |
Application Column
Primesep D
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsTriton X100
