| CAS Number | 55-56-1 |
|---|---|
| Molecular Formula | C22H30Cl2N10 |
| Molecular Weight | 505.451 |
| InChI Key | GHXZTYHSJHQHIJ-UHFFFAOYSA-N |
| LogP | 0.08 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Chlorhexidine in Biofluids: Blood Plasma and Urine on BIST B+ Column
November 30, 2022
HPLC Method for Analysis of Chlorhexidine Biofluids: Blood Plasma and Urine on BIST B+ by SIELC Technologies.
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analyses of Chlorhexidine
Chlorhexidine is a common antiseptic used intensively in hand sanitisers and other antibacterial products. Typical HPLC methods used to analyze this product are done in reverse phase (RP) mode, but this is complicated by the presence of two basic groups which, with the overall hydrophobic characteristics of the molecule, usually produce asymmetrical peaks.
Condition
| Column | BIST B+, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260 nm |
| Peak Retention Time | 6.2 min |
Description
| Class of Compounds | Topical antiseptic |
| Analyzing Compounds | Chlorhexidine |
Sample preparation:
| Plasma/urine sample was spiked with a small amount of Chlorhexidine and then diluted 5 times with acetonitrile. A formed precipitate was removed by centrifugation and the cleared solution was used directly for injection. |
Application Column
BIST B+
BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.
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HPLC Method for Analysis of Chlorhexidine on BIST B+ Column
November 30, 2022
HPLC Method for Analysis of Chlorhexidine on BIST B+ by SIELC Technologies.
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analyses of Chlorhexidine
Chlorhexidine is a common antiseptic used intensively in hand sanitisers and other antibacterial products. Typical HPLC methods used to analyze this product are done in reverse phase (RP) mode, but this is complicated by the presence of two basic groups which, with the overall hydrophobic characteristics of the molecule, usually produce asymmetrical peaks.
Condition
| Column | BIST B+, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260 nm |
| Peak Retention Time | 6.2 min |
Description
| Class of Compounds | Topical antiseptic |
| Analyzing Compounds | Chlorhexidine |
Application Column
BIST B+
BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.
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HPLC Method for Separation of Chlorhexidine and Cetylpyridinium Chloride on Primesep B Column
January 22, 2020
Chlorhexidine gluconate, or simply chlorhexidine, is a biguanide used as an antiseptic and disinfectant. It is a component of mouthwash rinses that has been shown to reduce plaque, gingivitis and oral bacteria. It’s also used as a topical agent for skin disinfection. Cetylpyridinium chloride is another type of antiseptic used in mouthwash rinses. Both compounds are cationic. They can be separated using HPLC on SIELC’s reverse-phase (RP) mixed-mode Primesep B column with the mobile phase of acetonitrile (ACN) and water with formic acid buffer and UV detected at 270nm.
| Column | Primesep B, 3.2×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | Formic Acid – 0.5% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 270 nm |
| Class of Compounds |
Surfactant, Hydrophobic, Ionizable |
| Analyzing Compounds | Chlorhexidine, Cetylpyridinium Chloride |
Application Column
Primesep B
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsChlorhexidine
Separation of Chlorhexidine on Newcrom R1 HPLC column
February 16, 2018
Chlorhexidine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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