Chlorhexidine

Chlorhexidine structural formula

CAS Number55-56-1
Molecular FormulaC22H30Cl2N10
Molecular Weight505.451
InChI KeyGHXZTYHSJHQHIJ-UHFFFAOYSA-N
LogP0.08
Synonyms
  • Chlorhexidine
  • N,N''''-Hexane-1,6-diylbis[N'-(4-chlorophenyl)triimidodicarbonic diamide]
  • Imidodicarbonimidic diamide, N,N''''-1,6-hexanediylbis[N'-(4-chlorophenyl)-
  • 55-56-1
  • 4-12-00-01201
  • 2,4,11,13-Tetraazatetradecanediimidamide, N,N''-bis(4-chlorophenyl)-3,12-diimino-
  • 1, 1'-Hexamethylenebis (5-(p-chlorophenyl) biguanide)
  • 1,1'-Hexamethylenebis[5-(p-chlorophenyl)biguanide]
  • 1,6-Bis[5-(p-chlorophenyl)biguanidino]hexane
  • 1,6-Di(N-p-chlorophenylbiguanidino)hexane
  • Biguanide, 1,1'-hexamethylenebis[5-(p-chlorophenyl)-
  • Chlorhexamed forte
  • Chlorhexidin
  • Chlorohex
  • Chlorohexidine
  • clorhexidina
  • Consepsis
  • Eludril
  • Hexadol
  • Hexident
  • Nolvasan
  • Soretol
  • TETRADECANE, 2,4,11,13-TETRAAZO, DIMIDAMIDE, N-N'BIS(4-CHLOROPHENYL)
  • Tubulicid
  • 1,6-Bis(5-(p-chlorophenyl)biguandino)hexane
  • 1,6-Bis(p-chlorophenyldiguanido)hexane
  • BRN 2826432
  • 1,6-Di(4'-chlorophenyldiguanido)hexane
  • EINECS 200-238-7
  • 1,1'-Hexamethylenebis(5-(p-chlorophenyl)biguanide)
  • Chlorhexidinum
  • Cloresidina
  • UNII-R4KO0DY52L
  • 1,1'-Hexamethylene bis(5-(p-chlorophenyl)biguanide)
  • Chlorhexidin [Czech]
  • Chlorhexidinum [INN-Latin]
  • Cloresidina [DCIT]
  • Clorhexidina [INN-Spanish]
  • N,N'-Bis(4-chlorophenyl)-3,12-diimino-2,4,11,13-tetraazatetradecanediimidamide
  • N-(4-chlorophenyl)-1-3-(6-{N-[3-(4-chlorophenyl)carbamimidamidomethanimidoyl]amino}hexyl)carbamimidamidomethanimidamide
  • 111883-36-4
  • 328933-19-3

Applications:

HPLC Method for Analysis of Chlorhexidine in Biofluids: Blood Plasma and Urine on BIST B+ Column

2022-11-30

HPLC Method for Analysis of Chlorhexidine Biofluids: Blood Plasma and Urine on BIST B+ by SIELC Technologies.

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Chlorhexidine in Biofluids Blood Plasma and Urine on BIST B+ Column
HPLC Method for Analysis of Chlorhexidine on BIST B+ by SIELC Technologies.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Chlorhexidine

Chlorhexidine is a common antiseptic used intensively in hand sanitisers and other antibacterial products. Typical HPLC methods used to analyze this product are done in reverse phase (RP) mode, but this is complicated by the presence of two basic groups which, with the overall hydrophobic characteristics of the molecule, usually produce asymmetrical peaks.


Condition

ColumnBIST B+, 4.6x150 mm, 5 µm, 100A
Mobile PhaseMeCN
BufferH2SO4 - 0.2%
Flow Rate1.0 ml/min
DetectionUV 260 nm
Peak Retention Time6.2 min

Description

Class of CompoundsTopical antiseptic
Analyzing CompoundsChlorhexidine

Sample preparation:

Plasma/urine sample was spiked with a small amount of Chlorhexidine and then diluted 5 times with acetonitrile. A formed precipitate was removed by centrifugation and the cleared solution was used directly for injection. 
SIELC Technologies usually develops more than one method for each compound on our website. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound of interest.
Application Analytes:
Chlorhexidine

HPLC Method for Analysis of Chlorhexidine on BIST B+ Column

2022-11-30

HPLC Method for Analysis of Chlorhexidine on BIST B+ by SIELC Technologies.

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Chlorhexidine on BIST B+ Column
HPLC Method for Analysis of Chlorhexidine on BIST B+ by SIELC Technologies.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Chlorhexidine

Chlorhexidine is a common antiseptic used intensively in hand sanitisers and other antibacterial products. Typical HPLC methods used to analyze this product are done in reverse phase (RP) mode, but this is complicated by the presence of two basic groups which, with the overall hydrophobic characteristics of the molecule, usually produce asymmetrical peaks.


Condition

ColumnBIST B+, 4.6x150 mm, 5 µm, 100A
Mobile PhaseMeCN
BufferH2SO4 - 0.2%
Flow Rate1.0 ml/min
DetectionUV 260 nm
Peak Retention Time6.2 min

Description

Class of CompoundsTopical antiseptic
Analyzing CompoundsChlorhexidine
SIELC Technologies usually develops more than one method for each compound on our website. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound of interest.
Application Analytes:
Chlorhexidine

HPLC Method for Separation of Chlorhexidine and Cetylpyridinium Chloride on Primesep B Column

2020-01-22
HPLC Separation of Chlorhexidine and Cetylpyridinium chloride on Primesep B Column_1222 Chlorhexidine gluconate, or simply chlorhexidine, is a biguanide used as an antiseptic and disinfectant. It is a component of mouthwash rinses that has been shown to reduce plaque, gingivitis and oral bacteria. It’s also used as a topical agent for skin disinfection. Cetylpyridinium chloride is another type of antiseptic used in mouthwash rinses. Both compounds are cationic. They can be separated using HPLC on SIELC’s reverse-phase (RP) mixed-mode Primesep B column with the mobile phase of acetonitrile (ACN) and water with formic acid buffer and UV detected at 270nm.

Condition

Column Primesep B,  3.2x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Formic Acid - 0.5%
Flow Rate 0.5 ml/min
Detection UV 270 nm

Description

Class of Compounds Surfactant,  Hydrophobic, Ionizable
Analyzing Compounds Chlorhexidine, Cetylpyridinium Chloride
SIELC Technologies usually develops more than one method for each compound on our website. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound of interest.
Application Analytes:
Cetylpyridinium Chloride
Chlorhexidine

Separation of Chlorhexidine on Newcrom R1 HPLC column

2018-02-16
Separation of Chlorhexidine on Newcrom C18 HPLC column

Chlorhexidine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com" or by phone: 847-229-2629.
SIELC Technologies usually develops more than one method for each compound on our website. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound of interest.
Application Analytes:
Chlorhexidine