HPLC Method for Analysis of Chlorhexidine Biofluids: Blood Plasma and Urine on BIST B+ by SIELC Technologies.
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analyses of Chlorhexidine
Chlorhexidine is a common antiseptic used intensively in hand sanitisers and other antibacterial products. Typical HPLC methods used to analyze this product are done in reverse phase (RP) mode, but this is complicated by the presence of two basic groups which, with the overall hydrophobic characteristics of the molecule, usually produce asymmetrical peaks.
Condition
| Column | BIST B+, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260 nm |
| Peak Retention Time | 6.2 min |
Description
| Class of Compounds | Topical antiseptic |
| Analyzing Compounds | Chlorhexidine |
Sample preparation:
| Plasma/urine sample was spiked with a small amount of Chlorhexidine and then diluted 5 times with acetonitrile. A formed precipitate was removed by centrifugation and the cleared solution was used directly for injection. |
Application Column
BIST B+
BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.
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