| CAS Number | 7439-93-2 |
|---|---|
| Molecular Formula | Li |
| Molecular Weight | 6.940 |
| InChI Key | SIAPCJWMELPYOE-UHFFFAOYSA-N |
| Synonyms |
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Applications:
HPLC Method for Separation of Sodium, Potassium, Lithium, and Ammonium on Primesep 100 Column
August 7, 2024
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ammonium Ion, Lithium, Potassium, Sodium on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ammonium Ion, Lithium, Potassium, Sodium
Inorganic cations and ammonia are usually analyzed by ion-exchange chromatography with a conductivity detector. Mixed-mode chromatography achieves the same goal with an ELSD detector. In addition to retaining ions, mixed-mode column can retain hydrophobic molecules also, making it possible to analyze a wide variety of ionic and non-ionic compounds in one run. In this method sodium, potassium, and lithium ions were separated along with ammonium ions on a Primesep 100 column. Detection technique is ELSD.
| Column | Primesep 100, 4.6 x 250 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 80/20% |
| Buffer | TFA – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
| Class of Compounds | Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Sodium, Potassium, Lithium, Ammonium |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Lithium
Potassium
Sodium
Reverse Elution Order in Separation of Alkali Cation
August 12, 2006
Primesep C separates a mixture of inorganic cations, potassium, sodium, and lithium, by a combination of cation exchange and complex formation. The peak order is reversed on the Primesep C column compared to the order normally obtained on columns that rely on cation exchange alone. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and ammonium acetate is compatible with UV and evaporative light scattering detection (ELSD).
| Column | Primesep C, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmAc |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD 50C |
| Class of Compounds |
Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Sodium, Potassium, Lithium, Chloride |
Application Column
Primesep C
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsPotassium
Sodium
Complex of Cations on Primesep C is Effected by Organic Modifier
October 15, 2005
Primesep C separates a mixture of inorganic cations, potassium, sodium, and lithium, by a combination of cation exchange and complex formation. The peak order is reversed on the Primesep C column compared to the order normally obtained on columns that rely on cation exchange alone. In addition, the elution order can be rearranged by simply changing the organic modifier in the mobile phase from acetonitrile to methanol. The mobile phase mixture of water, acetonitrile (MeCN, ACN) or methanol (MeOH) and ammonium acetate is compatible with UV and evaporative light scattering detection (ELSD).
| Column | Primesep C, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeOH, MeCN |
| Buffer | AmAc pH 5.0 |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD 50C |
| Class of Compounds |
Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Sodium, Potassium, Lithium, Chloride |
Application Column
Primesep C
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsPotassium
Sodium
HPLC ELSD Method for Separation of Metal Ions on Primesep 100 Column
October 4, 2005
Primesep 100 separates the monovalent cations, lithium, potassium, and sodium, and the divalent cations, zinc, manganese, and calcium. The cations are resolved by cation exchange. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) is evaporative light scattering detection (ELSD) compatible.
| Column | Primesep 100, 4.6×250 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 70/30% |
| Buffer | TFA- 0.4% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD 50C |
| Class of Compounds | Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Sodium, Magnesium, Calcium, Lithium, Barium |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Bromide
Calcium
Lithium
Magnesium
Potassium
Sodium
