Ammonium Ion

Ammonium Ion

CAS Number14798-03-9
Molecular FormulaH4N
Molecular Weight18.038
  • Ammonium
  • Ammonia, conjugate acid
  • 14798-03-9
  • Ammonia ion (NH4+)
  • Ammonia, ion (NH41+)
  • Ammonium (NH4+)
  • Ammonium cation
  • Ammonium ion
  • Ammonium ion (NH4+)
  • Ammonium ion(+)
  • Ammonium ion(1+)
  • Ammonium salt
  • Ammonium(1+)
  • UNII-54S68520I4
  • NH4(+)
  • [NH4](+)
  • 194419-49-3
  • 372952-69-7


Separation of Sodium, Potassium, Lithium, and Ammonium in Mixed-Mode HPLC


Separation type: Liquid Chromatography Mixed-mode

Inorganic cations and ammonia are usually analyzed by ion-exchange chromatography with a conductivity detector. Mixed-mode chromatography achieves the same goal with an ELSD detector. In addition to retaining ions, mixed-mode column can retain hydrophobic molecules also, making it possible to analyze a wide variety of ionic and non-ionic compounds in one run. In this method sodium, potassium, and lithium ions were separated along with ammonium ions on a Primesep 100 column. Detection technique is ELSD.


Column Primesep 100,  4.6×250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA
Flow Rate 1.0 ml/min
Detection ELSD



Class of Compounds
Ions,  Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Sodium, Potassium,  Bromide, Ammonium

Application Column

Primesep 100

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

Select options
Application Analytes:
Ammonium Ion

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound/s of interest.