HPLC Method for Separation of Sodium, Potassium, Lithium, and Ammonium on Primesep 100  Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Ammonium Ion, Lithium, Potassium, Sodium on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Ammonium Ion, Lithium, Potassium, Sodium on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Ammonium Ion, Lithium, Potassium, Sodium


Inorganic cations and ammonia are usually analyzed by ion-exchange chromatography with a conductivity detector. Mixed-mode chromatography achieves the same goal with an ELSD detector. In addition to retaining ions, mixed-mode column can retain hydrophobic molecules also, making it possible to analyze a wide variety of ionic and non-ionic compounds in one run. In this method sodium, potassium, and lithium ions were separated along with ammonium ions on a Primesep 100 column. Detection technique is ELSD.

ColumnPrimesep 100, 4.6 x 250 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 80/20%
BufferTFA – 0.1%
Flow Rate1.0 ml/min
DetectionELSD, the nebulizer and evaporator temperatures 50°C,
  with a gas flow rate of 1.6 Standard Liters per Minute (SLM)
Class of Compounds
Ions,  Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing CompoundsSodium, Potassium,  Lithium, Ammonium

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Ammonium Ion
Lithium
Potassium
Sodium
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.