Reverse Elution Order in Separation of Alkali Cation

Primesep C separates a mixture of inorganic cations, potassium, sodium, and lithium, by a combination of cation exchange and complex formation. The peak order is reversed on the Primesep C column compared to the order normally obtained on columns that rely on cation exchange alone. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and ammonium acetate is compatible with UV and evaporative light scattering detection (ELSD).


Column Primesep C, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc
Flow Rate 1.0 ml/min
Detection ELSD 50C


Class of Compounds Ions,  Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Sodium, Potassium,  Lithium, Chloride
Application Analytes: