Thiamine diphosphate (Thiamine pyrophosphate)

Thiamine diphosphate

CAS Number136-08-3
Molecular FormulaC12H19N4O7P2S+
Molecular Weight425.32
InChI KeyAYEKOFBPNLCAJY-UHFFFAOYSA-O
LogP-1.2
Synonyms
  • cocarboxylase
  • thiamine diphosphate
  • thiamin diphosphate
  • ThPP
  • thiamin pyrophosphate
  • ThDP
  • CHEBI:9532
  • thiamine-PPi
  • 136-08-3
  • thiamin-PPi
  • thiamine-pyrophosphate
  • thiamine(1+) diphosphate
  • 2-[3-[(4-amino-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-thiazol-3-ium-5-yl]ethyl phosphono hydrogen phosphate
  • NCGC00160617-01
  • 1ols
  • 1olu
  • 1olx
  • Thaimine pyrophosphate
  • 1v1m
  • AC1L1ASQ

Applications:

HPLC Method for Separation of 10 Water-Soluble Vitamins on Chromni Column

September 17, 2025

HPLC Method for Separation of 10 Water-Soluble Vitamins on Chromni™ by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for separation of Vitamin B2 (Riboflavin), Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), Nicotinamide, Vitamin B6 (Pyridoxine), Folic Acid, Cyanocobalamin, Thiamine diphosphate (Thiamine pyrophosphate), Vitamin B1 (Thiamine), Pantothenic Acid, Biotin

Riboflavin (B2), Nicotinic Acid (B3), Nicotinamide (B3), Pyridoxine (B6), Folic Acid (B9), Cyanocobalamin (B12), Thiamine Pyrophosphate (coenzyme of B1), Thiamine (B1), Pantothenic Acid (B5), Biotin (B7) are water soluble vitamins with a key function of energy metabolism. These coenzymes are responsible for converting food into usable energy.

Vitamin B2 (Riboflavin), Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), Nicotinamide, Vitamin B6 (Pyridoxine), Folic Acid, Cyanocobalamin, Thiamine diphosphate (Thiamine pyrophosphate), Vitamin B1 (Thiamine), Pantothenic Acid, Biotin can be retained and analyzed using the Chromni™ stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN). Detection is performed using UV.

ColumnChromni™, 4.6 x 150 mm, 3 µm, 100 A, dual ended
Mobile PhaseGradient of MeCN
BufferGradient of Phosphoric Acid
Flow Rate1.0 ml/min
DetectionUV 275 nm, 200 nm

Class of CompoundsVitamins
Analyzing CompoundsVitamin B2 (Riboflavin), Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), Nicotinamide, Vitamin B6 (Pyridoxine), Folic Acid, Cyanocobalamin, Thiamine diphosphate (Thiamine pyrophosphate), Vitamin B1 (Thiamine), Pantothenic Acid, Biotin

Application Column

Chromni™

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Biotin
Cyanocobalamin
Folic Acid
Nicotinamide
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Pantothenic Acid
Thiamine diphosphate (Thiamine pyrophosphate)
Vitamin B1 (Thiamine)
Vitamin B2 (Riboflavin)
Vitamin B6 (Pyridoxine)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of Thiamine Pyrophosphate on PEI Column

December 7, 2020


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Condition
Column PEI, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN -50%,
Buffer H3PO4 – 1.0%
Flow Rate 1.0 ml/min
Detection UV 250 nm

 

Description
Class of Compounds
 Vitamin, Drug
Analyzing Compounds Thiamine Pyrophosphate

 

Application Column

PEI

SIELC Technologies offers custom phases for customers who require specific separations not achievable with commercially available HPLC phases. Considering the vast array of compounds and mixtures requiring analysis, tailored LC phases can significantly enhance separation results for unique and challenging applications. To learn more about our special custom LC phases designed for your specific separation needs, please contact SIELC Technologies at research@sielc.com. Our team of experts is ready to guide you through the process and create a custom solution that addresses your particular chromatographic challenges.

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Application Analytes:
Thiamine diphosphate (Thiamine pyrophosphate)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Thiamine Pyrophosphate and Pyridoxal Phosphate on Primesep SB Column

July 11, 2017

Condition
Column Primesep SB, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmFm pH 3.0
Flow Rate 1.0 ml/min
Detection UV, 290 nm

 

Description
Class of Compounds
 Hydrophilic, Ionizable
Analyzing Compounds Thiamine Pyrophosphate, Pyridoxal Phosphate

 

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Pyridoxal Phosphate
Thiamine diphosphate (Thiamine pyrophosphate)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.