HPLC Method for Analysis of Thiamine Pyrophosphate and Pyridoxal Phosphate on Primesep SB Column


Column Primesep SB, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmFm pH 3.0
Flow Rate 1.0 ml/min
Detection UV, 290 nm


Class of Compounds Hydrophilic, Ionizable
Analyzing Compounds Thiamine Pyrophosphate, Pyridoxal Phosphate
Application Analytes:
Pyridoxal Phosphate
Thiamine diphosphate (Thiamine pyrophosphate)