HPLC Method for Analysis of Thiamine Pyrophosphate and Pyridoxal Phosphate on Primesep SB Column

Condition
Column Primesep SB, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmFm pH 3.0
Flow Rate 1.0 ml/min
Detection UV, 290 nm

 

Description
Class of Compounds
 Hydrophilic, Ionizable
Analyzing Compounds Thiamine Pyrophosphate, Pyridoxal Phosphate

 

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Pyridoxal Phosphate
Thiamine diphosphate (Thiamine pyrophosphate)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.