Procainamide

Procainamide structural formula

CAS Number51-06-9
Molecular FormulaC13H21N3O
Molecular Weight235.331
InChI KeyREQCZEXYDRLIBE-UHFFFAOYSA-N
LogP0.88
Synonyms
  • Procainamide
  • 4-Amino-N-[2-(diethylamino)ethyl]benzamide
  • Benzamide, 4-amino-N-[2-(diethylamino)ethyl]-
  • 51-06-9
  • 4-14-00-01154
  • Benzamide, 4-amino-N-[2-(diethylamino)ethyl]-
  • 4-Amino-N-(diethylaminoethyl)benzamide
  • 4-Amino-N-[2-(diethylamino)ethyl]benzamide
  • Benzamide, p-amino-N-[2-(diethylamino)ethyl]-
  • Novocainamid
  • Novocainamide
  • Novocaine amide
  • Novocamid
  • NSC 27461
  • p-Aminobenzoic diethylaminoethylamide
  • p-Amino-N-(2-diethylaminoethyl)benzamide
  • Procainamid
  • procainamida
  • Procaine amide
  • Procanbid
  • 4-Amino-N-(2-(diethylamino)ethyl)benzamide
  • Benzamide, 4-amino-N-(2-(diethylamino)ethyl)-
  • Biocoryl
  • BRN 2214285
  • EINECS 200-078-8
  • Procamide
  • 2-Diethylaminoethylamid kyseliny p-aminobenzoove
  • Procainamidum
  • UNII-L39WTC366D
  • Rhythmin

Applications:

HPLC MS Method for Analysis of Procainamide on Primesep 200 Column

February 15, 2024

HPLC MS Method for Analysis of Procainamide on Primesep 200 Column

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of Procainamide on Primesep 200 Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Procainamide

Procainamide is a medication that belongs to the class of antiarrhythmic drugs. It is used to treat certain types of irregular heartbeats (arrhythmias), particularly those associated with atrial and ventricular fibrillation or atrial flutter. Procainamide works by stabilizing the heart’s electrical activity and helping to restore a normal rhythm.

Procainamide blocks sodium channels in the heart, which helps to slow down the conduction of electrical impulses. By doing so, it can prevent abnormal electrical activity in the heart.

Procainamide can be retained and analyzed using a Primesep 200 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and ammonium formate as a buffer. This method allows for detection using UV at 280 nm

ColumnPrimesep 200, 2.1 x 100 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN 30-60%, 10 min
BufferAmFm pH 3.0  – 10 mM
Flow Rate0.2 ml/min
DetectionUV 280nm, SIM + 236
Samples0.15mg/ml
Injection volume5 µl
LOD*30 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Antiarrhythmic agent, Amide
Analyzing CompoundsProcainamide

Application Column

Primesep 200

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Procainamide

Application Detection:
UV Detection
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Separation of Procainamide and Procaine on BIST B+ Column Column

February 12, 2024

High Performance Liquid Chromatography (HPLC) Method for Analysis of Procainamide, Procaine on BIST B+ by SIELC Technologies

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Separation of Procainamide and Procaine on BIST B+ Column Column
HPLC Method for Analysis of Procainamide, Procaine on on BIST B+ by SIELC Technologies

Procainamide and procaine are two different pharmaceutical compounds with distinct uses and properties.

Procainamide:

Class: Procainamide is an antiarrhythmic medication, belonging to the Class Ia antiarrhythmic drugs. It is used to treat certain types of abnormal heart rhythms (arrhythmias) by stabilizing the cell membrane and reducing excitability in the heart muscle.
Mechanism of Action: Procainamide works by inhibiting sodium channels in cardiac cells, prolonging the action potential duration and refractory period, which helps in controlling irregular heartbeats.
Administration: It is typically administered intravenously or orally under medical supervision.
Medical Use: Procainamide is prescribed for conditions such as atrial and ventricular arrhythmias.
Procaine:

Class: Procaine is a local anesthetic drug, belonging to the amino ester group of local anesthetics.
Mechanism of Action: Procaine works by blocking nerve impulses and causing temporary loss of sensation in a specific area of the body.
Administration: It is usually administered through injection to induce local anesthesia. Procaine is commonly used for minor surgical procedures and dental work.
Medical Use: Procaine is employed for local anesthesia, providing pain relief in various medical and dental settings.
Procainamide is primarily used for cardiac conditions, while procaine is utilized as a local anesthetic for pain management during medical and dental procedures. While both compounds have a shared ancestry in PABA and contain amine groups, the specific functional groups (amide vs. ester) and their positions in the molecules contribute to their distinct pharmacological properties. It’s a good observation that small structural changes can lead to significant differences in the pharmacological actions of compounds.

Procainamide and procaine can be retained, separated and analyzed on a BIST B+ mixed-mode stationary phase column using an analytical method with a simple mobile phase of water, Acetonitrile (MeCN) , and a sulfuric acid as a buffer. This analysis method can be detected using UV at 280 nm.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Procainamide, Procaine

Condition

ColumnBIST B+, 4.6 x 50 mm, 5 µm, 100 A
Mobile Phase MeCN/H2O – 80/20%
Buffe H2SO4 -0.1%
Flow Rate1.0 ml/min
DetectionUV 280 nm
Sample1.  0.14 mg/ml
2.  0.15 mg/ml
LOD *1. 2 ppb
2. 5 ppb
Injection volume2 µl

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed

Description

Class of CompoundsDrug
Analyzing CompoundsProcainamide, Procaine

Application Column

BIST B+

Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Procainamide
Procaine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Separation of Procainamide and Procaine on Newcrom A Column

February 6, 2024

HPLC Method for Analysis of Procainamide, Procaine on Newcrom A by SIELC Technologies

HPLC Method for Separation of Procainamide and Procaine on Primesep B Column
HPLC Method for Analysis of Procainamide, Procaine on Newcrom A Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Procainamide, Procaine

Procainamide and procaine are two different pharmaceutical compounds with distinct uses and properties.

Procainamide:

  • Class: Procainamide is an antiarrhythmic medication, belonging to the Class Ia antiarrhythmic drugs. It is used to treat certain types of abnormal heart rhythms (arrhythmias) by stabilizing the cell membrane and reducing excitability in the heart muscle.
  • Mechanism of Action: Procainamide works by inhibiting sodium channels in cardiac cells, prolonging the action potential duration and refractory period, which helps in controlling irregular heartbeats.
  • Administration: It is typically administered intravenously or orally under medical supervision.
  • Medical Use: Procainamide is prescribed for conditions such as atrial and ventricular arrhythmias.

Procaine:

  • Class: Procaine is a local anesthetic drug, belonging to the amino ester group of local anesthetics.
  • Mechanism of Action: Procaine works by blocking nerve impulses and causing temporary loss of sensation in a specific area of the body.
  • Administration: It is usually administered through injection to induce local anesthesia. Procaine is commonly used for minor surgical procedures and dental work.
  • Medical Use: Procaine is employed for local anesthesia, providing pain relief in various medical and dental settings.

Procainamide is primarily used for cardiac conditions, while procaine is utilized as a local anesthetic for pain management during medical and dental procedures. While both compounds have a shared ancestry in PABA and contain amine groups, the specific functional groups (amide vs. ester) and their positions in the molecules contribute to their distinct pharmacological properties. It’s a good observation that small structural changes can lead to significant differences in the pharmacological actions of compounds.

 Procainamide and Procaine can be retained, separated and analyzed using a Newcrom A mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and phosphoricacid as a buffer. Detection is achieved using UV 280 nm

ColumnNewcrom A, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O -20/80%
BufferH3PO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 280 nm

Class of Compounds
Drug
Analyzing CompoundsProcainamide, Procaine

Application Column

Newcrom A

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Procainamide
Procaine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

USP Methods for the Separation of Propranolol and Procainamide using a Legacy L1 Column

June 21, 2012

 

Application Notes:
Propranolol is a common beta-blocker used for treating anxiety and hypertension, and procainamide is an antiarrhythmic drug. According to the USP methods, propranolol hydrochloride contain no less than 98% and no more than 101.5 percent of propranolol calculated on a dried basis. The USP HPLC method for the separation of phenylephrine and epinephrine was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of USP.

Application Columns: Legacy L1 C18 HPLC column

Application compounds: Propranolol and procainamide

Mobile phase: Water/MeOH/MeCN (70/70/90) with 7mM sodium lauryl sulfate and 11 mM phosphoric acid

Detection technique: UV

Reference: USP35: NF30

Condition

Column Legacy L1, 4.6×150 mm, 5 µm, 100A
Mobile Phase Water/MeOH/MeCN (70/70/90) with 7mM Sodium Lauryl   sulfate and 11   mM Phosphoric acid
Buffer Sodium Lauryl   Sulfate, Phosphoric acid
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Drug, Beta blocker, Antiarrhythmic, Hydrophobic, Ionizable, Neutral
Analyzing Compounds Propranolol, Procainamide

 

Application Column

SIELC Legacy L1 HPLC column

Legacy L1

SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.

Select options
Application Analytes:

Procainamide
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

USP Methods Analysis of Procainamide on Legacy L1 Column

June 21, 2012

 


Application Columns: Legacy L1 C18 HPLC column

Application compounds: Procainamide and 4-aminobenzoic acid

Mobile phase: WAter/MeOH/MeCN (70/70/90) with 7 mM sodium lauryl sulfate and 11mM phosphoric acid

Detection technique: UV

Reference: USP35: NF30

Condition

Column Legacy L1, 4.6×150 mm, 5 µm, 100A
Mobile Phase Water/MeOH/TEA (140/60/1)   adjusted to pH 7.5 with H3PO4
Buffer TEAPh
Flow Rate 1.5 ml/min
Detection UV, 230 nm

 

Description

Class of Compounds
Drug, Nonsteroidal anti-inflammatory drug, Hydrophobic, Ionizable
Analyzing Compounds Ibuprofen, benzophenone

 

Application Column

SIELC Legacy L1 HPLC column

Legacy L1

SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.

Select options
Application Analytes:
4-Aminobenzoic Acid
Procainamide
Procainamide Hydrochloride

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.