USP Methods Analysis of Procainamide on Legacy L1 Column

 


Application Columns: Legacy L1 C18 HPLC column

Application compounds: Procainamide and 4-aminobenzoic acid

Mobile phase: WAter/MeOH/MeCN (70/70/90) with 7 mM sodium lauryl sulfate and 11mM phosphoric acid

Detection technique: UV

Reference: USP35: NF30

Condition
Column Legacy L1, 4.6×150 mm, 5 µm, 100A
Mobile Phase Water/MeOH/TEA (140/60/1)   adjusted to pH 7.5 with H3PO4
Buffer TEAPh
Flow Rate 1.5 ml/min
Detection UV, 230 nm

 

Description
Class of Compounds
 Drug, Nonsteroidal anti-inflammatory drug, Hydrophobic, Ionizable
Analyzing Compounds Ibuprofen, benzophenone

 

Application Column

SIELC Legacy L1 HPLC column

Legacy L1

SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.

Select options
Application Analytes:
4-Aminobenzoic Acid
Procainamide
Procainamide Hydrochloride
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.