USP Methods Analysis of Procainamide Legacy L1 Column


Application Columns: Legacy L1 C18 HPLC column

Application compounds: Procainamide and 4-aminobenzoic acid

Mobile phase: WAter/MeOH/MeCN (70/70/90) with 7 mM sodium lauryl sulfate and 11mM phosphoric acid

Detection technique: UV

Reference: USP35: NF30


Column Legacy L1, 4.6×150 mm, 5 µm, 100A
Mobile Phase Water/MeOH/TEA (140/60/1)   adjusted to pH 7.5 with H3PO4
Buffer TEAPh
Flow Rate 1.5 ml/min
Detection UV, 230 nm



Class of Compounds
Drug, Nonsteroidal anti-inflammatory drug, Hydrophobic, Ionizable
Analyzing Compounds Ibuprofen, benzophenone


Application Column

SIELC Legacy L1 HPLC column

Legacy L1

SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.

Select options
Application Analytes:
4-Aminobenzoic Acid
Procainamide Hydrochloride

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound/s of interest.