Propranolol is a common beta-blocker used for treating anxiety and hypertension, and procainamide is an antiarrhythmic drug. According to the USP methods, propranolol hydrochloride contain no less than 98% and no more than 101.5 percent of propranolol calculated on a dried basis. The USP HPLC method for the separation of phenylephrine and epinephrine was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of USP.
Legacy L1 C18 HPLC column
Propranolol and procainamide
Water/MeOH/MeCN (70/70/90) with 7mM sodium lauryl sulfate and 11 mM phosphoric acid
||Legacy L1, 4.6x150 mm, 5 µm, 100A
||Water/MeOH/MeCN (70/70/90) with 7mM Sodium Lauryl sulfate and 11 mM Phosphoric acid
||Sodium Lauryl Sulfate, Phosphoric acid
||UV, 270 nm
|Class of Compounds
||Drug, Beta blocker, Antiarrhythmic, Hydrophobic, Ionizable, Neutral
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