HPLC MS Method for Analysis of Procainamide on Primesep 200 Column

HPLC MS Method for Analysis of Procainamide on Primesep 200 Column by SIELC Technologies

HPLC Method for Analysis of Procainamide on Primesep 200 Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Procainamide

Procainamide is an antiarrhythmic medication with the chemical formula C13H21N3O. It belongs to the Class Ia antiarrhythmic drugs. It is used to treat certain types of abnormal heart rhythms (arrhythmias) by stabilizing the cell membrane and reducing excitability in the heart muscle. Procainamide works by inhibiting sodium channels in cardiac cells, prolonging the action potential duration and refractory period, which helps in controlling irregular heartbeats. It is typically administered intravenously or orally under medical supervision. You can find detailed UV spectra of Procainamide and information about its various lambda maxima by visiting the following link.

Procainamide can be retained and analyzed using a [comlumn] mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and ammonium formate as a buffer. This method allows for detection using UV at 280 nm

ColumnPrimesep 200, 2.1 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseGradient MeCN 30-60%, 10 min
BufferAmFm pH 3.0  – 10 mM
Flow Rate0.2 ml/min
DetectionUV 280nm, SIM + 236
Samples0.15mg/ml
Injection volume5 µl
LOD*30 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Class of Compounds
Antiarrhythmic agent, Amide
Analyzing CompoundsProcainamide

Application Column

Primesep 200

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Procainamide
Application Detection:
UV Detection
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.