USP Methods for the Separation of Propranolol and Procainamide using a Legacy L1 Column


Application Notes:
Propranolol is a common beta-blocker used for treating anxiety and hypertension, and procainamide is an antiarrhythmic drug. According to the USP methods, propranolol hydrochloride contain no less than 98% and no more than 101.5 percent of propranolol calculated on a dried basis. The USP HPLC method for the separation of phenylephrine and epinephrine was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of USP.

Application Columns: Legacy L1 C18 HPLC column

Application compounds: Propranolol and procainamide

Mobile phase: Water/MeOH/MeCN (70/70/90) with 7mM sodium lauryl sulfate and 11 mM phosphoric acid

Detection technique: UV

Reference: USP35: NF30


Column Legacy L1, 4.6×150 mm, 5 µm, 100A
Mobile Phase Water/MeOH/MeCN (70/70/90) with 7mM Sodium Lauryl   sulfate and 11   mM Phosphoric acid
Buffer Sodium Lauryl   Sulfate, Phosphoric acid
Flow Rate 1.0 ml/min
Detection UV, 270 nm



Class of Compounds
Drug, Beta blocker, Antiarrhythmic, Hydrophobic, Ionizable, Neutral
Analyzing Compounds Propranolol, Procainamide


Application Column

SIELC Legacy L1 HPLC column

Legacy L1

SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.

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Application Analytes:

SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound/s of interest.