2,4,5-Trichlorophenoxyacetic acid

2,4,5-Trichlorophenoxyacetic acid structural formula

CAS Number93-76-5
Molecular FormulaC8H5Cl3O3
Molecular Weight255.480
InChI KeySMYMJHWAQXWPDB-UHFFFAOYSA-N
LogP3.31
Synonyms
  • 2,4,5-Trichlorophenoxyacetic acid
  • (2,4,5-Trichlorophenoxy)acetic acid
  • Acetic acid, 2-(2,4,5-trichlorophenoxy)-
  • 93-76-5
  • 2,4,5-T
  • 4-06-00-00973
  • Acetic acid, (2,4,5-trichlorophenoxy)-
  • (2,4,5-Trichlorophenoxy)acetic acid
  • 2,4,5-Trichlorophenoxyacetate
  • Acetic acid, 2-(2,4,5-trichlorophenoxy)-
  • Arbokan
  • BCF-Bushkiller
  • NSC 430
  • Trichlorophenoxyacetic acid
  • Trioxon
  • BRN 2055620
  • Brush Killer
  • Brush RHAP
  • Brush-off 445 low volatile brush killer
  • Brushtox
  • Caswell No. 881
  • Crossbow
  • Decamine 4T
  • Ded-Weed
  • Ded-weed brush killer
  • Ded-weed lv-6 brush kil and t-5 brush kil
  • EINECS 202-273-3
  • Envert-T
  • EPA Pesticide Chemical Code 082001
  • Estercide t-2 and t-245
  • Farmco fence rider
  • Fence rider
  • Fruitone A
  • Inverton 245
  • Line rider
  • Phortox
  • RCRA waste number U232
  • Spontox
  • Super D Weedone
  • Tippontormona
  • Visko Rhap
  • Visko rhap low volatile ester
  • (2,4,5-Trichloor-fenoxy)-azijnzuur
  • (2,4,5-Trichlor-phenoxy)-essigsaeure
  • Acide 2,4,5-trichloro phenoxyacetique
  • Acido (2,4,5-tricloro-fenossi)-acetico
  • Cido (2,4,5-tricloro-fenossi)-acetico
  • Kwas 2,4,5-trojchlorofenoksyoctowy
  • UNII-9Q963S4YMX
  • (2,4,5-Trichlorphenoxy)essigsaeure
  • 2,4,5-Trichlorphenoxyessigsaeure
  • 2-(2,4,5-trichlorophenoxy)acetic acid
  • 2-(2,4,5-trichlorophenoxy)ethanoic acid
  • Esteron 245
  • Trioxone

Applications:

HPLC Separation of Pesticides, Herbicides, Fungicides and Insecticides on Newcrom BH Column

May 22, 2020


Herbicides are used to control unwanted plants, they are also known as weedkillers. Insecticides are used to kill insects. Fungicides are used to kill parasitic fungi. Pesticide is a more generic term that includes herbicides, fungicides and insecticides in its definition. All are heavily used in agriculture. By using HPLC, many different pesticides can be separated and their retention characteristics controlled using the Newcrom BH mixed-mode column. It can be used with different organic media such as acetonitrile (ACN) or methanol (MeOH). By varying the concentration of organic modifier and using different buffers like sulfuric acid (H2SO4), ammonium formate (AmFm), or formic acid, separation of desired pesticides can be achieved.

Condition

Column Newcrom BH, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN, MeOH
Buffer H2SO4, Formic acid, AmFm pH 3.0
Flow Rate 0.6 ml/min
Detection UV 275 nm

Description

Class of Compounds Pesticides, Herbicides, Fungicides, Insecticides
Analyzing Compounds Monocrotophos, Metribuzin, Bromacil, Carbofuran, 4-Chlorophenoxyacetic acid, Bentazone, Folpet, Fenthion, Fenvalerate, Metrabuzin, Carbofuran, Atrazine, Parathion –methyl, MCPA, Clopyralid, Dicamba, Benthazon, Simazine, Diuron, MCPB, Mecoprop, 4-Chlorophenoxyacetic acid,2,4,5-T

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
2,4,5-Trichlorophenoxyacetic acid
4-CPA
Atrazine
Bentazone-sodium
Carbofuran
Clopyralid
Dicamba
Diuron
Fenvalerate
Folpet
MCPA
MCPB
Mecoprop
Methyl parathion
Metribuzin
Monocrotophos
Simazine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Analysis of 2,4,5-T on Mixed-Mode Columns

September 14, 2015

Separation type: Liquid Chromatography Mixed-mode



 

2,4,5-T is a synthetic herbicide that defoliates broad-leaf plants. It was a major component of Agent Orange, a defoliant used during the Vietnam War. 2,4,5-T has been replaced by dicamba and triclopyr due to its possible toxicity. 2,4,5-T was included in a proficiency test ran by the EURL (European Union Reference Laboratory) using QuEChERS methodology on acidic pesticides. Obelisc R retained 2,4,5-T because it has a long hydrophobic chain and ionic pairing groups. Primesep 100 retains by reverse-phase and with acid ion-pairing groups. Method is LC/MS compatible and same conditions can be used on many different pesticides.

Condition 1

Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 25-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm

 

Condition 2

Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm

 

Condition 3

Column Primesep 100, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds 2,4,5-T

 

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
2,4,5-Trichlorophenoxyacetic acid

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Analysis of 2,4,5-Trichlorophenoxyacetic acid on Primesep B2 Column

August 24, 2015

 

 

2,4,5-Trichlorophenoxyacetic acid (2,4,5-T) is a synthetic auxin and herbicide that was widely used until the 1970s. The EURL (European Union Reference Laboratory) included a QuEChERS (Quick, Easy, Cheap, Rugged, and Safe) method for 2,4,5-T as a target pesticide for EUPT-SRM. Obelisc R and Primesep B2 were used to retain 2,4,5-T and separate it from impurities. Primesep B2 contains embedded basic ion-pairing groups while Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. This method is LC/MS compatible and can be used as a general approach for analyzing 2,4,5-T and dozens of other pesticides.

 

Condition 

Column Primesep B2, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 4.5- 20-50 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm

 

 

Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds 2,4,5-Trichlorophenoxyacetic acid

 

Application Column

Primesep B2

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
2,4,5-Trichlorophenoxyacetic acid

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Analysis of 2,4,5-Trichlorophenoxyacetic acid on Obelisc R Column

June 12, 2015

 

 

2,4,5-Trichlorophenoxyacetic acid (2,4,5-T) is a synthetic auxin and herbicide that was widely used until the 1970s. The EURL (European Union Reference Laboratory) included a QuEChERS (Quick, Easy, Cheap, Rugged, and Safe) method for 2,4,5-T as a target pesticide for EUPT-SRM. Obelisc R and Primesep B2 were used to retain 2,4,5-T and separate it from impurities. Primesep B2 contains embedded basic ion-pairing groups while Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. This method is LC/MS compatible and can be used as a general approach for analyzing 2,4,5-T and dozens of other pesticides.

 

Condition 

Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 4.5- 20-50 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm

 

 

Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds 2,4,5-Trichlorophenoxyacetic acid

 

Application Column

Obelisc R

Column Diameter: 2.1 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
2,4,5-Trichlorophenoxyacetic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.