Metribuzin

Metribuzin structural formula

CAS Number21087-64-9
Molecular FormulaC8H14N4OS
Molecular Weight214.290
InChI KeyFOXFZRUHNHCZPX-UHFFFAOYSA-N
LogP1.54
Synonyms
  • Metribuzin
  • 4-Amino-6-tert-butyl-3-(methylsulfanyl)-1,2,4-triazin-5(4H)-one
  • 1,2,4-Triazin-5(4H)-one, 4-amino-6-(1,1-dimethylethyl)-3-(methylthio)-
  • 21087-64-9
  • 5-26-06-00432
  • 1,2,4-Triazin-5(4H)-one, 4-amino-6-(1,1-dimethylethyl)-3-(methylthio)-
  • 3-Methylthio-4-amino-6-tert-butyl-1,2,4-triazin-5(4H)-one
  • 3-Methylthio-4-amino-6-tert-butyl-1,2,4-triazin-5-one
  • 4-Amino-6-(1,1-dimethylethyl)-3-(methylthio)-1,2,4-triazin-5(4H)-one
  • 4-Amino-6-tert-butyl-3-(methylthio)-1,2,4-triazin-5(4H)-one
  • 4-Amino-6-tert-butyl-3-(methylthio)-1,2,4-triazin-5-one
  • 4-Amino-6-tert-butyl-3-(methylthio)-1,2,4-triazine-5(4H)-one
  • 4-Amino-6-tert-butyl-3-(methylthio)-4,5-dihydro-1,2,4-triazin-5-one
  • 4-Amino-6-tert-butyl-3-(methylthio)-as-triazin-5(4H)-one
  • 4-Amino-6-tert-butyl-4,5-dihydro-3-methylthio-1,2,4-triazin-5-one
  • as-Triazin-5(4H)-one, 4-amino-6-tert-butyl-3-(methylthio)-
  • Lexone DF
  • Metribuzine
  • Sencor 75DF
  • Sencorex
  • Sencorex L.F.
  • Zontran
  • 4-Amino-6-tert-butyl-3-methylthio-1,2,4-triazin-5(4H)-one
  • BAY dic 1468
  • Bayer 6159H
  • Bayer 6443H
  • Bayer 94337
  • BRN 0746650
  • Caswell No. 033D
  • EINECS 244-209-7
  • EPA Pesticide Chemical Code 101101
  • Sencor DF
  • 1,2,4-Triazin-5-one, 4-amino-6-tert-butyl-3-(methylthio)-
  • UNII-QO836138OV

Applications:

HPLC Separation of Pesticides, Herbicides, Fungicides and Insecticides on Newcrom BH Column

May 22, 2020


Herbicides are used to control unwanted plants, they are also known as weedkillers. Insecticides are used to kill insects. Fungicides are used to kill parasitic fungi. Pesticide is a more generic term that includes herbicides, fungicides and insecticides in its definition. All are heavily used in agriculture. By using HPLC, many different pesticides can be separated and their retention characteristics controlled using the Newcrom BH mixed-mode column. It can be used with different organic media such as acetonitrile (ACN) or methanol (MeOH). By varying the concentration of organic modifier and using different buffers like sulfuric acid (H2SO4), ammonium formate (AmFm), or formic acid, separation of desired pesticides can be achieved.

Condition

Column Newcrom BH, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN, MeOH
Buffer H2SO4, Formic acid, AmFm pH 3.0
Flow Rate 0.6 ml/min
Detection UV 275 nm

Description

Class of Compounds Pesticides, Herbicides, Fungicides, Insecticides
Analyzing Compounds Monocrotophos, Metribuzin, Bromacil, Carbofuran, 4-Chlorophenoxyacetic acid, Bentazone, Folpet, Fenthion, Fenvalerate, Metrabuzin, Carbofuran, Atrazine, Parathion –methyl, MCPA, Clopyralid, Dicamba, Benthazon, Simazine, Diuron, MCPB, Mecoprop, 4-Chlorophenoxyacetic acid,2,4,5-T

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
2,4,5-Trichlorophenoxyacetic acid
4-CPA
Atrazine
Bentazone-sodium
Carbofuran
Clopyralid
Dicamba
Diuron
Fenvalerate
Folpet
MCPA
MCPB
Mecoprop
Methyl parathion
Metribuzin
Monocrotophos
Simazine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Metribuzin on Mixed-Mode HPLC Columns

September 9, 2015

Separation type: Liquid Chromatography Mixed-mode



 

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Metribuzin.
Metribuzin is a pre- and post- emergence herbicide on the EURL (European Union Reference Laboratory)  Target Pesticide List for the EUPT-CF9 2015 (European Union Proficiency Test for Cereals and Feeding stuff). Metribuzin disrupts photosynthesis in plants growing near food crops such as potatoes, tomatoes and sugar came. Obelisc R and Primesep 100 were used to retain Metribuzin using multiple modes of separation. Obelisc R contains a long hydrophobic chain and ion-pairing groups, while Primesep 100 contains embedded acidic ion-pairing groups. Method is LC/MS compatible and can be used with similar conditions to retain many pesticides.

 

Condition 1

Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 25-70%, 15 min
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm

 

Condition 2

Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm

 

Condition 3

Column Primesep 100, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Metribuzin

 

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Metribuzin

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.