2,4,5-Trichlorophenoxyacetic acid (2,4,5-T) is a synthetic auxin and herbicide that was widely used until the 1970s. The EURL (European Union Reference Laboratory) included a QuEChERS (Quick, Easy, Cheap, Rugged, and Safe) method for 2,4,5-T as a target pesticide for EUPT-SRM. Obelisc R and Primesep B2 were used to retain 2,4,5-T and separate it from impurities. Primesep B2 contains embedded basic ion-pairing groups while Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. This method is LC/MS compatible and can be used as a general approach for analyzing 2,4,5-T and dozens of other pesticides.
| Column | Primesep B2, 2.1×150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
| Buffer | Gradient AmAc pH 4.5- 20-50 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | 2,4,5-Trichlorophenoxyacetic acid |
Application Column
Primesep B2
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsApplication Analytes:
2,4,5-Trichlorophenoxyacetic acidApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
