HPLC Method for Analysis of Vitamin B1 (Thiamine) on Chromni Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Vitamin B1 (Thiamine).

Thiamine, also known as vitamin B1, is an essential nutrient with the chemical formula C₁₂H₁₇ClN₄OS. It plays a crucial role in energy metabolism and the proper functioning of the nervous system. It helps the body convert carbohydrates into energy and is vital for the growth, development, and function of cells. You can find detailed UV spectra of Vitamin B1 (Thiamine) and information about its various lambda maxima by visiting the following link.

Vitamin B1 (Thiamine) can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using LC MS.

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Description

HPLC Method for Separation of 8 Water-Soluble Vitamins on Chromni by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for separation of Vitamin B2 (Riboflavin), Nicotinic Acid (Vitamin B3), Vitamin B6 (Pyridoxine), Folic Acid, Cyanocobalamin, Thiamine diphosphate (Thiamine pyrophosphate), Vitamin B1 (Thiamine), Pantothenic Acid (Vitamin B5), Biotin

Riboflavin (B2), Nicotinic Acid (B3), Folic Acid (B9), Cyanocobalamin (B12), Thiamine Pyrophosphate (coenzyme of B1), Thiamine (B1), Pantothenic Acid (B5), Biotin (B7) are water soluble vitamins with a key function of energy metabolism. These coenzymes are responsible for converting food into usable energy.

Pantothenic Acid, also known as vitamin B5, is an essential nutrient with the chemical formula C₁₉H₁₇NO₅. It plays a crucial role in energy and coenzyme A production. That energy and coenzyme are used for synthesis and degradation of proteins, carbohydrates, and fats. You can find detailed UV spectra of Pantothenic Acid (Vitamin B5) and information about its various lambda maxima by visiting the following link.

Biotin (Vitamin B7) is a water-soluble vitamin with the chemical formula C₁₀H₁₆N₂O₃S. It helps the body breakdown key nutrients in order for it to absorb and utilize them. A lack of biotin can lead to hair loss, rash, and brittle nails. A healthy diet including eggs, milk, and bananas can supply the body with a healthy dose of biotin. You can find detailed UV spectra of Biotin and information about its various lambda maxima by visiting the following link.

Nicotinic acid, also known as niacin and vitamin B3, is a water-soluble B vitamin with C₆H₅NO₂ molecular formula. It is an essential nutrient that is crucial for energy production. Nicotinic acid is commonly used as a dietary supplement to treat vitamin B3 deficiency, and it’s also prescribed in higher doses to help lower cholesterol levels. It works by dilating blood vessels, which can lead to a flushing sensation, hence it is sometimes referred to as “flushing niacin.” You can find detailed UV spectra of Niacin and information about its various lambda maxima by visiting the following link.

Riboflavin, also known as Vitamin B2, is an essential vitamin with the chemical formula C₁₇H₂₀N₄O₆. It is crucial in the formation of flavin mononucleotide and flavin adenine dinucleotide. Those coenzyme are involved in a large variety of tasks, including but not limited to energy metabolism, cellular respiration, antibody production, and metabolism of niacin and folate. It is also used as yellow food coloring. You can find detailed UV spectra of Riboflavin and information about its various lambda maxima by visiting the following link.

Folic Acid, also known as vitamin B9, folic acid is crucial for many functions in the body, including DNA synthesis and repair, cell division, and growth. As an essential vitamin, Folic acid must be obtained through diet or supplementation, as humans cannot synthesize it. Vitamin B-9 is a crucial prenatal vitamin. that also helps the body use iron properly. It has the chemical formula C₁₉H₁₉N₇O₆. You can find detailed UV spectra of Folic Acid and information about its various lambda maxima by visiting the following link.

Thiamine, also known as vitamin B1, is an essential nutrient with the chemical formula C₁₂H₁₇ClN₄OS. It plays a crucial role in energy metabolism and the proper functioning of the nervous system. It helps the body convert carbohydrates into energy and is vital for the growth, development, and function of cells. You can find detailed UV spectra of Vitamin B1 (Thiamine) and information about its various lambda maxima by visiting the following link.

Cyanocobalamin, also known as vitamin B12, is an essential nutrient with the chemical formula C₆₃H₈₈CoN₁₄O₁₄P. It is naturally present in some foods and available as a dietary supplement and as prescription medication. Vitamin B12 exists in several forms and contains the mineral cobalt, so compounds with vitamin B12 activity are collectively called “cobalamins”. It plays an essential role in the production of the red blood cells and DNA, as well as in the proper functioning of the nervous system. You can find detailed UV spectra of Cyanocobalamin and information about its various lambda maxima by visiting the following link.

Thiamine Pyrophosphate, an active form of  Vitamin B1, is a vitamin with the chemical formula C₁₂H₁₉N₄O₇P₂S⁺. B1 is a water soluble vitamin with a key function of energy metabolism. It is not produced in the body naturally and is stored in very small amounts; therefore must be regularly consumed through diet of whole rains, nuts, seeds, and legumes. It is vital as a coenzyme in carbohydrate metabolism and in decarboxylation of pyruvate, alpha-ketoglutarate, and branched-chain keto acids. You can find detailed UV spectra of Thiamine and information about its various lambda maxima by visiting the following link.

Vitamin B2 (Riboflavin), Nicotinic Acid (Vitamin B3), Vitamin B6 (Pyridoxine), Folic Acid, Cyanocobalamin, Thiamine diphosphate (Thiamine pyrophosphate), Vitamin B1 (Thiamine), Pantothenic Acid (Vitamin B5), Biotin can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN). Detection is performed using UV.

Gradient Elution Program for HPLC Method

High Performance Liquid Chromatography (HPLC) Method for Analysis of Water-Soluble Vitamins (B1, B2, B3, B6) on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Vitamin B1 (Thiamine), Vitamin B2 (Riboflavin), Nicotinamide, Vitamin B6 (Pyridoxine), Pyridoxamine, Pyridoxal Phosphate

Water-Soluble Vitamins (B1, B2, B3, B6) can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an gradient method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 300 nm.

You can find detailed UV spectra of Vitamin B1 (Thiamine), Vitamin B2 (Riboflavin), Nicotinamide, Vitamin B6 (Pyridoxine), Pyridoxamine, Pyridoxal Phosphate and information about its various lambda maxima by visiting the following links: Vitamins (B1, B2, B3, B6)

High Performance Liquid Chromatography (HPLC) Method for Analysis of Vitamin B1 (Thiamine) on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Vitamin B1 (Thiamine)

Thiamine, also known as vitamin B1, is an essential nutrient that plays a crucial role in energy metabolism and the proper functioning of the nervous system. It helps the body convert carbohydrates into energy and is vital for the growth, development, and function of cells.

Vitamin B1 (Thiamine) can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 250 nm.

You can find detailed UV spectra of Vitamin B1 (Thiamine) and information about its various lambda maxima by visiting the following link.

Access the UV-Vis Spectrum SIELC Library

UV-Vis Spectrum of Thiamine. Absorption Maxima: 246 nm.

If you are looking for optimized HPLC method to analyze Vitamin B1 (Thiamine) check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Separation of vitamins group B is achieved on Obelisc N column in HILIC mixed-mode. Vitamins of this group are different in polarity and ionic properties. Retention and separation is achieved by optimization of amount of ACN, buffer and buffer pH. Combination of UV and ELSD detection is used to monitor HPLC separation. B vitamins are water-soluble vitamins that play an important role in cell metabolism. Supplements containing all six are generally referred to as a vitamin B complex. Individual B vitamin supplements are referred to by the specific name of each vitamin. This method can be used to analyze individual B vitamins as well as vitamin B complex. Isolation of impurities as well as degradation products is possible by preparative chromatography.

Primesep C separates B vitamins with baseline resolution by a combination of cation exchange, complex formation, and hydrophobic interactions. Vitamin B1 (thiamine), vitamin B2 (riboflavin), and vitamin B6 (pyridoxine) are separated with a mobile phase of water, acetonitrile (MeCN, ACN) and triethylamine (TEA) phosphate with UV detection at 280 nm.