HPLC LC MS Method for Analysis of Thiamine on Chromni Column

HPLC Method for Analysis of Vitamin B1 (Thiamine) on Chromni Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Vitamin B1 (Thiamine).

Thiamine, also known as vitamin B1, is an essential nutrient with the chemical formula C12H17ClN4OS. It plays a crucial role in energy metabolism and the proper functioning of the nervous system. It helps the body convert carbohydrates into energy and is vital for the growth, development, and function of cells. You can find detailed UV spectra of Vitamin B1 (Thiamine) and information about its various lambda maxima by visiting the following link.

Vitamin B1 (Thiamine) can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using LC MS.

Condition

ColumnChromni, 2.1 x 150 mm, 3 µm, 100 A, surface coated
Mobile PhaseGradient MeCN/H2O – 70/30 – 40/60%, 10 min
BufferAmmonium formate pH 3.0 – 20 mM
Flow Rate0.2 ml/min
DetectionESI-SIM: [M+2H]2+ 265.1
Limit Of Detection*4 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsVitamin
Analyzing CompoundsVitamin B1 (Thiamine)

Application Column

Chromni

Column Diameter: 2.1 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: surface coated

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Application Analytes:
Vitamin B1 (Thiamine)
Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.