HPLC Method for Oxalic Acid on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Oxalic Acid.

Oxalic Acid is an organic acid with the chemical formula C₂H₂O₄. It is considered the simplest dicarboxylic acid, with it’s name being derrived from the genus of the plant from which it was isolated, Oxalis. It is primarily used as a bleaching agent in cleaning solutions, rust removal, and teeth whitening. Oxalic acid has an oral lowest published lethal dose of 600 mg/kg. You can find detailed UV spectra of Oxalic Acid and information about its various lambda maxima by visiting the following link.

Multi-charged molecules, such as oxaloacetate and oxalate, generally have a tendency to exhibit poor peak shape on reverse-phase HPLC columns, where they show significant tailing. Oxalic Acid can be retained with great peak shape on a Newcrom BH mixed-mode column. Oxalic acid can be measured at low UV. Using a Newcrom BH mixed-mode column and a mobile phase consisting of acetonitrile (ACN) and water with sulfuric acid (H2SO4) buffer, oxaloacetic acid and oxalic acid can be retained, separated, and UV detected at 200 nm with precise resolution.

If you are looking for optimized HPLC method to analyze Oxalic Acid check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

HPLC Method for Analysis of Oxalic Acid, Glycolic acid, Acetic Acid, Glyoxylic acid on Newcrom BH by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

1. Glyoxylic Acid (CHO-COOH): This is the smallest α-keto acid. It is a yellowish solid that occurs naturally and is useful industrially. It participates in many biochemical reactions. Its reduced form, D-lactic acid, can be found in various microorganisms.
2. Glycolic Acid (HOCH2COOH): This is the smallest α-hydroxy acid (AHA). It’s derived from sugar cane and is a colorless, odorless, and hygroscopic crystalline solid. It is used in various skin-care products because of its capability to penetrate skin.
3. Acetic Acid (CH3COOH): It is a simple carboxylic acid. It’s an important chemical reagent and industrial chemical, primarily used in the production of cellulose acetate for photographic film and polyvinyl acetate for wood glue, as well as synthetic fibers and fabrics. It gives vinegar its sour taste and pungent smell.
4. Oxalic Acid (C2H2O4): This is a colorless crystalline solid that forms a colorless solution in water. It occurs naturally in many foods, but too much can lead to kidney stones. It’s used in many different industrial processes, including rust removal, bleaching, and as a reducing agent in developing photographic film.

These four acids be separate and analyzed on a reverse-phase Newcrom BH, 4.6 x 150 mm, 3 µm, 100 A, dual ended column with a mobile phase consisting of water and perchloric acid as a buffer modifier. This analysis method can be UV detected at 200 nm.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Oxalic Acid, Glycolic acid, Acetic Acid, Glyoxylic acid

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HPLC Method for Maleic Acid, Ascorbic Acid, Nicotinic Acid (Vitamin B3), Fumaric Acid, Oxalic Acid on BIST A+ by SIELC Technologies

Maleic Acid, also known as cis-butenedioic acid,  is a dicarboxylic acid with the chemical formula C₄H₄O₄. It is primarily used as a precursor to fumaric acid.  It is is derived by hydrolysis of maleic anhydride or is produced by oxidation of benzene or butane.  In medicine, it is used to form salts with drugs to increase their stability. You can find detailed UV spectra of Maleic Acid and information about its various lambda maxima by visiting the following link.

Ascorbic Acid is a vitamin with the molecular formula C₆H₈O₆. Typically, it is used to treat scurvy, support immune system, and preserve food. It is a white to light yellow powder that is easily dissolved in water. It can be found in a large variety of fruits and vegetables, especially in citrus. You can find detailed UV spectra of Ascorbic Acid and information about its various lambda maxima by visiting the following link.

Nicotinic acid, also known as niacin and vitamin B3, is a water-soluble B vitamin with C₆H₅NO₂ molecular formula. It is an essential nutrient that is crucial for energy production. Nicotinic acid is commonly used as a dietary supplement to treat vitamin B3 deficiency, and it’s also prescribed in higher doses to help lower cholesterol levels. It works by dilating blood vessels, which can lead to a flushing sensation, hence it is sometimes referred to as “flushing niacin.” You can find detailed UV spectra of Niacin and information about its various lambda maxima by visiting the following link.

Fumaric Acid, also known as trans-butenedioic acid, is an organic compound with C4H4O4 chemical formula. It is used across food, industrial, and medical industries. In food, is it often used as a preservative, pH regulator, and flavoring akin to citric acid.  Industrially, it is used in making polyester resins, polyhydric alcohols, and more. Medically, it is used in denture cleaners and it’s derivatives are used in treating psoriasis. Fumaric acid is a popular preservative and food additive with a fruit-like taste. You can find detailed UV spectra of Fumaric Acid and information about its various lambda maxima by visiting the following link.

Oxalic Acid is an organic acid with the chemical formula C₂H₂O₄. It is considered the simplest dicarboxylic acid, with it’s name being derrived from the genus of the plant from which it was isolated, Oxalis. It is primarily used as a bleaching agent in cleaning solutions, rust removal, and teeth whitening. Oxalic acid has an oral lowest published lethal dose of 600 mg/kg. You can find detailed UV spectra of Oxalic Acid and information about its various lambda maxima by visiting the following link.

Using SIELC’s newly introduced BIST™ method, a mixture of these organic acids can be separated on a negatively-charged, cation-exchange BIST A+ column, contrary to conventional chromatographic wisdom. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include Calcium acetate and Magnesium acetate. Using this new and unique analysis method, these organic acids can be separated, retained, and detected through ELSD. This method is also compatible with Mass Spectrometry (LC-MS) and CAD.

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HPLC Method for Oxalic Acid on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Oxalic Acid.

Oxalic Acid is an organic acid with the chemical formula C₂H₂O₄. It is considered the simplest dicarboxylic acid, with it’s name being derrived from the genus of the plant from which it was isolated, Oxalis. It is primarily used as a bleaching agent in cleaning solutions, rust removal, and teeth whitening. Oxalic acid has an oral lowest published lethal dose of 600 mg/kg. You can find detailed UV spectra of Oxalic Acid and information about its various lambda maxima by visiting the following link.

Multi-charged molecules, such as oxaloacetate and oxalate, generally have a tendency to exhibit poor peak shape on reverse-phase HPLC columns, where they show significant tailing. Oxalic Acid can be retained with great peak shape on a Newcrom BH mixed-mode column. Oxalic acid can be measured at low UV. Using a Newcrom BH mixed-mode column and a mobile phase consisting of acetonitrile (ACN) and water with sulfuric acid (H2SO4) buffer, oxaloacetic acid and oxalic acid can be retained, separated, and UV detected at 200 nm with precise resolution.

HPLC Method for Oxalic Acid on Newcrom B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Oxalic Acid 

Oxalic Acid. also known as ethanedioic acid is an organic acid. It occurs naturally in plants, but is also often found in industrial uses as a bleaching agent, rust remover, and cleaning agent. Industrial uses of it also extend into households, as it is easily accessible. Despite that, in large amounts, it can be toxic.

Oxalic Acid can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using CAD.