| CAS Number | 2552-55-8 |
|---|---|
| Molecular Formula | C5H6N2O4 |
| Molecular Weight | 158.11 |
| InChI Key | IRJCBFDCFXCWGO-UHFFFAOYSA-N |
| LogP | -3.9 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Ibotenic Acid
January 12, 2026
If you are looking for optimized HPLC method to analyze Ibotenic acid check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Analysis of Ibotenic Acid on Primesep A Column
February 28, 2023
HPLC Method for Analysis of Ibotenic acid on Primesep A by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Ibotenic acid is a physchoactive NDMA and metabotropic glutamate receptor agonist with neurotoxic properties. These structurally similar psychoactive drugs can be retained, separated, and analyzed on a mixed-mode Primesep A column with a mobile phase consisting of water, Acetonitrile (MeCN), and Phosphoric acid (H3PO4). This analytical method can be UV detected at 210 nm with high resolution and peak symmetry.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ibotenic Acid
Condition
| Column | Primesep A, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 5/95% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Peak Retention Time | 4.12 min |
Description
| Class of Compounds | Acid |
| Analyzing Compounds | Ibotenic acid |
Application Column
Primesep A
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
HPLC Method for Separation of Ibotenic Acid and Glutamic Acid on Primesep A Column
February 28, 2023
HPLC Method for Separation of Ibotenic Acid and Glutamic Acid on Primesep A by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Ibotenic acid is a physchoactive NDMA and metabotropic glutamate receptor agonist with neurotoxic properties. These structurally similar psychoactive drugs can be retained, separated, and analyzed on a mixed-mode Primesep A column with a mobile phase consisting of water, Acetonitrile (MeCN), and Phosphoric acid (H3PO4). This analytical method can be UV detected at 210 nm with high resolution and peak symmetry.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ibotenic Acid and Glutamic Acid
Condition
| Column | Primesep A, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 5/95% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Peak Retention Time | 3.22 min, 10.52 min |
Description
| Class of Compounds | Acid |
| Analyzing Compounds | Ibotenic Acid, Glutamic Acid |
Application Column
Primesep A
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
GLU (L-Glutamic acid)
Glutamic Acid
Ibotenic acid
L-Glutamic acid hydrochloride
HPLC Method for Separation of Ibotenic Acid and Muscimol on a Primesep 100 Column
February 24, 2023
HPLC Method for Separation of Ibotenic Acid and Muscimol on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Ibotenic acid is a physchoactive NDMA and metabotropic glutamate receptor agonist with neurotoxic properties. Muscimol (also known as agarin or pantherine) is a psychoactive GABA-A agonist. Both are naturally occurring compounds found in Amanita muscaria and related species of mushrooms. These structurally similar psychoactive drugs can be retained, separated, and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (H2SO4). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ibotenic Acid and Muscimol
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H2SO4 – 0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Peak Retention Time | 2.22 min, 8.52 min |
Description
| Class of Compounds | Acid |
| Analyzing Compounds | Ibotenic Acid, Muscimol |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Muscimol
