| CAS Number | 2552-55-8 |
|---|---|
| Molecular Formula | C5H6N2O4 |
| Molecular Weight | 158.11 |
| InChI Key | IRJCBFDCFXCWGO-UHFFFAOYSA-N |
| LogP | -3.9 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Ibotenic Acid
January 12, 2026
If you are looking for optimized HPLC method to analyze Ibotenic acid check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Analysis of Ibotenic Acid on Primesep A Column
February 28, 2023
HPLC Method for Analysis of Ibotenic acid on Primesep A by SIELC Technologies
Ibotenic acid, also known as ibotenate, premuscimol or as (S)-2-amino-2-(3-hydroxyisoxazol-5-yl)acetic acid, is an a-amino acid with the chemical formula C5H6N2O4. It is a metabotropic glutamate receptor agonist found in certain Amanita mushrooms. It is known for it’s neurotoxic properties. You can find detailed UV spectra of Ibotenic acid and information about its various lambda maxima by visiting the following link.
Ibotenic acid can be retained, separated, and analyzed on a mixed-mode Ibotenic acid column with a mobile phase consisting of water, Acetonitrile (MeCN), and Phosphoric acid (H3PO4). This analytical method can be UV detected at 210 nm with high resolution and peak symmetry.
Condition
| Column | Primesep A, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 5/95% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Peak Retention Time | 4.12 min |
Description
| Class of Compounds | Acid |
| Analyzing Compounds | Ibotenic acid |
Application Column
Primesep A
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
HPLC Method for Separation of Ibotenic Acid and Glutamic Acid on Primesep A Column
February 28, 2023
HPLC Method for Separation of Ibotenic Acid and Glutamic Acid on Primesep A by SIELC Technologies
Ibotenic acid, also known as ibotenate, premuscimol or as (S)-2-amino-2-(3-hydroxyisoxazol-5-yl)acetic acid, is an a-amino acid with the chemical formula C5H6N2O4. It is a metabotropic glutamate receptor agonist found in certain Amanita mushrooms. It is known for it’s neurotoxic properties. You can find detailed UV spectra of Ibotenic acid and information about its various lambda maxima by visiting the following link.
Glutamic Acid, also known as Glu or E, is an a-amino acid with the chemical formula C5H9NO4. It is used by almost all organisms for biosynthesis of protein and cellular metabolism. It also serves as a precursor for the synthesis of the inhibitory gamma-aminobutyric acid in GABAergic neurons. It is known to be responsible for the umami flavor and is used as a flavor enhancer. You can find detailed UV spectra of Ibotenic acid, Glutamic Acid, GLU (L-Glutamic acid), D-Glutamic acid, L-Glutamic acid hydrochloride and information about its various lambda maxima by visiting the following link.
Ibotenic acid, Glutamic Acid, GLU (L-Glutamic acid), D-Glutamic acid, L-Glutamic acid hydrochloride can be retained, separated, and analyzed on a mixed-mode Primesep A column with a mobile phase consisting of water, Acetonitrile (MeCN), and Phosphoric acid (H3PO4). This analytical method can be UV detected at 210 nm with high resolution and peak symmetry.
Condition
| Column | Primesep A, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 5/95% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Peak Retention Time | 3.22 min, 10.52 min |
Description
| Class of Compounds | Acid |
| Analyzing Compounds | Ibotenic acid, Glutamic Acid, GLU (L-Glutamic acid), D-Glutamic acid, L-Glutamic acid hydrochloride |
Application Column
Primesep A
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
GLU (L-Glutamic acid)
Glutamic Acid
Ibotenic acid
L-Glutamic acid hydrochloride
HPLC Method for Separation of Ibotenic Acid and Muscimol on a Primesep 100 Column
February 24, 2023
HPLC Method for Separation of Muscimol, Ibotenic acid on Primesep 100 by SIELC Technologies
Ibotenic acid, also known as ibotenate, premuscimol or as (S)-2-amino-2-(3-hydroxyisoxazol-5-yl)acetic acid, is an a-amino acid with the chemical formula C5H6N2O4. It is a metabotropic glutamate receptor agonist found in certain Amanita mushrooms. It is known for it’s neurotoxic properties. You can find detailed UV spectra of Ibotenic acid and information about its various lambda maxima by visiting the following link.
Muscimol, also known as agarin, pantherine, and pyroibotenic acid, is a psychoactive GABA-A agonist with the chemical formula C4H6N2O2. It is found in Amanita mushrooms. Muscimol is well known for its psychoactive, hallucinogenic, and sedative properties. You can find detailed UV spectra of Muscimol and information about its various lambda maxima by visiting the following link.
Muscimol, Ibotenic acid can be retained, separated, and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (H2SO4). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry.
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H2SO4 – 0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Peak Retention Time | 2.22 min, 8.52 min |
Description
| Class of Compounds | Acid |
| Analyzing Compounds | Muscimol, Ibotenic acid |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Muscimol
