| CAS Number | 6893-26-1 |
|---|---|
| Molecular Formula | C5H9NO4 |
| Molecular Weight | 147.131 |
| InChI Key | WHUUTDBJXJRKMK-GSVOUGTGSA-N |
| LogP | -2.77 |
| Synonyms |
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Applications:
HPLC Method for Separation of Ibotenic Acid and Glutamic Acid on Primesep A Column
February 28, 2023
HPLC Method for Separation of Ibotenic Acid and Glutamic Acid on Primesep A by SIELC Technologies
Ibotenic acid, also known as ibotenate, premuscimol or as (S)-2-amino-2-(3-hydroxyisoxazol-5-yl)acetic acid, is an a-amino acid with the chemical formula C5H6N2O4. It is a metabotropic glutamate receptor agonist found in certain Amanita mushrooms. It is known for it’s neurotoxic properties. You can find detailed UV spectra of Ibotenic acid and information about its various lambda maxima by visiting the following link.
Glutamic Acid, also known as Glu or E, is an a-amino acid with the chemical formula C5H9NO4. It is used by almost all organisms for biosynthesis of protein and cellular metabolism. It also serves as a precursor for the synthesis of the inhibitory gamma-aminobutyric acid in GABAergic neurons. It is known to be responsible for the umami flavor and is used as a flavor enhancer. You can find detailed UV spectra of Ibotenic acid, Glutamic Acid, GLU (L-Glutamic acid), D-Glutamic acid, L-Glutamic acid hydrochloride and information about its various lambda maxima by visiting the following link.
Ibotenic acid, Glutamic Acid, GLU (L-Glutamic acid), D-Glutamic acid, L-Glutamic acid hydrochloride can be retained, separated, and analyzed on a mixed-mode Primesep A column with a mobile phase consisting of water, Acetonitrile (MeCN), and Phosphoric acid (H3PO4). This analytical method can be UV detected at 210 nm with high resolution and peak symmetry.
Condition
| Column | Primesep A, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 5/95% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Peak Retention Time | 3.22 min, 10.52 min |
Description
| Class of Compounds | Acid |
| Analyzing Compounds | Ibotenic acid, Glutamic Acid, GLU (L-Glutamic acid), D-Glutamic acid, L-Glutamic acid hydrochloride |
Application Column
Primesep A
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
GLU (L-Glutamic acid)
Glutamic Acid
Ibotenic acid
L-Glutamic acid hydrochloride
Separation of D-Glutamic acid on Newcrom R1 HPLC column
February 16, 2018
D-Glutamic acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options