HPLC Method for Separation of Ibotenic Acid and Glutamic Acid on Primesep A by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Ibotenic acid is a physchoactive NDMA and metabotropic glutamate receptor agonist with neurotoxic properties. These structurally similar psychoactive drugs can be retained, separated, and analyzed on a mixed-mode Primesep A column with a mobile phase consisting of water, Acetonitrile (MeCN), and Phosphoric acid (H3PO4). This analytical method can be UV detected at 210 nm with high resolution and peak symmetry.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ibotenic Acid and Glutamic Acid
Condition
| Column | Primesep A, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 5/95% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Peak Retention Time | 3.22 min, 10.52 min |
Description
| Class of Compounds | Acid |
| Analyzing Compounds | Ibotenic Acid, Glutamic Acid |
Application Column
Primesep A
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
D-Glutamic acidGLU (L-Glutamic acid)
Glutamic Acid
Ibotenic acid
L-Glutamic acid hydrochloride
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
