HPLC Method for Separation of Ibotenic Acid and Glutamic Acid on Primesep A by SIELC Technologies

Ibotenic acid, also known as ibotenate, premuscimol or as (S)-2-amino-2-(3-hydroxyisoxazol-5-yl)acetic acid, is an a-amino acid with the chemical formula C₅H₆N₂O₄. It is a metabotropic glutamate receptor agonist found in certain Amanita mushrooms. It is known for it’s neurotoxic properties. You can find detailed UV spectra of Ibotenic acid and information about its various lambda maxima by visiting the following link.

Glutamic Acid, also known as Glu or E, is an a-amino acid with the chemical formula C₅H₉NO₄. It is used by almost all organisms for biosynthesis of protein and cellular metabolism. It also serves as a precursor for the synthesis of the inhibitory gamma-aminobutyric acid in GABAergic neurons. It is known to be responsible for the umami flavor and is used as a flavor enhancer. You can find detailed UV spectra of Ibotenic acid, Glutamic Acid, GLU (L-Glutamic acid), D-Glutamic acid, L-Glutamic acid hydrochloride and information about its various lambda maxima by visiting the following link.

Ibotenic acid, Glutamic Acid, GLU (L-Glutamic acid), D-Glutamic acid, L-Glutamic acid hydrochloride can be retained, separated, and analyzed on a mixed-mode Primesep A column with a mobile phase consisting of water, Acetonitrile (MeCN), and Phosphoric acid (H3PO4). This analytical method can be UV detected at 210 nm with high resolution and peak symmetry.

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