HPLC Method for Separation of Ibotenic Acid and Muscimol on a Primesep 100 Column

HPLC Method for Separation of Muscimol, Ibotenic acid on Primesep 100 by SIELC Technologies

HPLC Method for Separation of Ibotenic Acid and Muscimolon Primesep 100 Column by SIELC Technologies
Method for Separation of Muscimol, Ibotenic acid on Primesep 100 by SIELC Technologies

Ibotenic acid, also known as ibotenate, premuscimol or as (S)-2-amino-2-(3-hydroxyisoxazol-5-yl)acetic acid, is an a-amino acid with the chemical formula C5H6N2O4. It is a metabotropic glutamate receptor agonist found in certain Amanita mushrooms. It is known for it’s neurotoxic properties. You can find detailed UV spectra of Ibotenic acid and information about its various lambda maxima by visiting the following link.

Muscimol, also known as agarin, pantherine, and pyroibotenic acid, is a psychoactive GABA-A agonist with the chemical formula C4H6N2O2. It is found in Amanita mushrooms. Muscimol is well known for its psychoactive, hallucinogenic, and sedative properties. You can find detailed UV spectra of Muscimol and information about its various lambda maxima by visiting the following link.

Muscimol, Ibotenic acid can be retained, separated, and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (H2SO4). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry.

Condition

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 20/80%
BufferH2SO4 – 0.05%
Flow Rate1.0 ml/min
DetectionUV 200 nm
Peak Retention Time2.22 min, 8.52 min

Description

Class of CompoundsAcid
Analyzing CompoundsMuscimol, Ibotenic acid

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Ibotenic acid
Muscimol
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.