| CAS Number | 2763-96-4 |
|---|---|
| Molecular Formula | C4H6N2O2 |
| Molecular Weight | 114.104 |
| InChI Key | ZJQHPWUVQPJPQT-UHFFFAOYSA-N |
| LogP | -2.39 |
| Synonyms |
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Applications:
HPLC Method for Separation of Muscimol and Thiomuscimol Primesep 100 Column
February 27, 2023
HPLC Method for Separation of Muscimol and Thiomuscimol on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Muscimol (also known as agarin or pantherine) is a psychoactive GABA-A agonist. It is a naturally occurring compound found in Amanita muscaria and related species of mushrooms. Thiomuschimol is also a GABA-A agonist with a near identical structure as Muscimol with the Oxygen in the ring replaced with a Sulfur. These structurally similar psychoactive drugs can be retained, separated, and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Ammonium formate (AmFm). This analytical method can be UV detected at 250 nm with high resolution and peak symmetry.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Muscimol and Thiomuscimol
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | Ammonium Formate pH 4.0- 40 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250 nm |
| Peak Retention Time | 5.22 min, 6.52 min |
Description
| Class of Compounds | Isoxazole, Heterocyclic |
| Analyzing Compounds | Muscimol, Thiomuscimol |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Thiomuscimol
HPLC Method for Separation of Ibotenic Acid and Muscimol on a Primesep 100 Column
February 24, 2023
HPLC Method for Separation of Ibotenic Acid and Muscimol on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Ibotenic acid is a physchoactive NDMA and metabotropic glutamate receptor agonist with neurotoxic properties. Muscimol (also known as agarin or pantherine) is a psychoactive GABA-A agonist. Both are naturally occurring compounds found in Amanita muscaria and related species of mushrooms. These structurally similar psychoactive drugs can be retained, separated, and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (H2SO4). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ibotenic Acid and Muscimol
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H2SO4 – 0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Peak Retention Time | 2.22 min, 8.52 min |
Description
| Class of Compounds | Acid |
| Analyzing Compounds | Ibotenic Acid, Muscimol |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Muscimol
Separation of Muscimol on Newcrom R1 HPLC column
February 16, 2018
Muscimol can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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