CAS Number | 62020-54-6 |
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Molecular Formula | C4H6N2OS |
Molecular Weight | 130.17 |
InChI Key | QVBUOPGWPXUAHT-UHFFFAOYSA-N |
LogP | -1.3 |
Synonyms |
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Applications:
HPLC Method for Separation of Muscimol and Thiomuscimol Primesep 100 Column
February 27, 2023
HPLC Method for Separation of Muscimol and Thiomuscimol on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Muscimol (also known as agarin or pantherine) is a psychoactive GABA-A agonist. It is a naturally occurring compound found in Amanita muscaria and related species of mushrooms. Thiomuschimol is also a GABA-A agonist with a near identical structure as Muscimol with the Oxygen in the ring replaced with a Sulfur. These structurally similar psychoactive drugs can be retained, separated, and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Ammonium formate (AmFm). This analytical method can be UV detected at 250 nm with high resolution and peak symmetry.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Muscimol and Thiomuscimol
Condition
Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
Mobile Phase | MeCN/H2O – 20/80% |
Buffer | Ammonium Formate pH 4.0- 40 mM |
Flow Rate | 1.0 ml/min |
Detection | UV 250 nm |
Peak Retention Time | 5.22 min, 6.52 min |
Description
Class of Compounds | Isoxazole, Heterocyclic |
Analyzing Compounds | Muscimol, Thiomuscimol |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Thiomuscimol