| CAS Number | 62020-54-6 |
|---|---|
| Molecular Formula | C4H6N2OS |
| Molecular Weight | 130.17 |
| InChI Key | QVBUOPGWPXUAHT-UHFFFAOYSA-N |
| LogP | -1.3 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Thiomuscimol
January 12, 2026
If you are looking for optimized HPLC method to analyze Thiomuscimol check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Separation of Muscimol and Thiomuscimol Primesep 100 Column
February 27, 2023
HPLC Method for Separation of Muscimol and Thiomuscimol on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Muscimol (also known as agarin or pantherine) is a psychoactive GABA-A agonist. It is a naturally occurring compound found in Amanita muscaria and related species of mushrooms. Thiomuschimol is also a GABA-A agonist with a near identical structure as Muscimol with the Oxygen in the ring replaced with a Sulfur. These structurally similar psychoactive drugs can be retained, separated, and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Ammonium formate (AmFm). This analytical method can be UV detected at 250 nm with high resolution and peak symmetry.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Muscimol and Thiomuscimol
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | Ammonium Formate pH 4.0- 40 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250 nm |
| Peak Retention Time | 5.22 min, 6.52 min |
Description
| Class of Compounds | Isoxazole, Heterocyclic |
| Analyzing Compounds | Muscimol, Thiomuscimol |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Thiomuscimol
