HPLC Method for Analysis of Ibotenic Acid on Primesep A Column

HPLC Method for Analysis of Ibotenic acid on Primesep A by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Ibotenic Acid on Primesep A by SIELC Technologies.
HPLC Method for Analysis of Ibotenic acid on Primesep A by SIELC Technologies

Ibotenic acid is a physchoactive NDMA and metabotropic glutamate receptor agonist with neurotoxic properties. These structurally similar psychoactive drugs can be retained, separated, and analyzed on a mixed-mode Primesep A column with a mobile phase consisting of water, Acetonitrile (MeCN), and Phosphoric acid (H3PO4). This analytical method can be UV detected at 210 nm with high resolution and peak symmetry.

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Ibotenic Acid

Condition

ColumnPrimesep A, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 5/95%
BufferH3PO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 210 nm
Peak Retention Time4.12 min

Description

Class of CompoundsAcid
Analyzing CompoundsIbotenic acid

Application Column

Primesep A

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Ibotenic acid

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.