HPLC Method for Analysis of Ibotenic Acid on Primesep A Column

HPLC Method for Analysis of Ibotenic acid on Primesep A by SIELC Technologies

HPLC Method for Analysis of Ibotenic Acid on Primesep A by SIELC Technologies.
HPLC Method for Analysis of Ibotenic acid on Primesep A by SIELC Technologies

Ibotenic acid, also known as ibotenate, premuscimol or as (S)-2-amino-2-(3-hydroxyisoxazol-5-yl)acetic acid, is an a-amino acid with the chemical formula C5H6N2O4. It is a metabotropic glutamate receptor agonist found in certain Amanita mushrooms. It is known for it’s neurotoxic properties. You can find detailed UV spectra of Ibotenic acid and information about its various lambda maxima by visiting the following link.

Ibotenic acid can be retained, separated, and analyzed on a mixed-mode Ibotenic acid column with a mobile phase consisting of water, Acetonitrile (MeCN), and Phosphoric acid (H3PO4). This analytical method can be UV detected at 210 nm with high resolution and peak symmetry.

Condition

ColumnPrimesep A, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 5/95%
BufferH3PO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 210 nm
Peak Retention Time4.12 min

Description

Class of CompoundsAcid
Analyzing CompoundsIbotenic acid

Application Column

Primesep A

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Ibotenic acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.