HPLC Method for Analysis of Ibotenic Acid on Primesep A Column

HPLC Method for Analysis of Ibotenic acid on Primesep A by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

Ibotenic acid is a physchoactive NDMA and metabotropic glutamate receptor agonist with neurotoxic properties. These structurally similar psychoactive drugs can be retained, separated, and analyzed on a mixed-mode Primesep A column with a mobile phase consisting of water, Acetonitrile (MeCN), and Phosphoric acid (H3PO4). This analytical method can be UV detected at 210 nm with high resolution and peak symmetry.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Ibotenic Acid

Condition

Column	Primesep A, 4.6 x 150 mm, 5 µm, 100 A
Mobile Phase	MeCN/H2O – 5/95%
Buffer	H3PO4 – 0.1%
Flow Rate	1.0 ml/min
Detection	UV 210 nm
Peak Retention Time	4.12 min

Description

Class of Compounds	Acid
Analyzing Compounds	Ibotenic acid

Application Column

Primesep A

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Ibotenic acid

UV Detection

SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

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