| CAS Number | 493-52-7 |
|---|---|
| Molecular Formula | C15H15N3O2 |
| Molecular Weight | 269.304 |
| InChI Key | CEQFOVLGLXCDCX-WUKNDPDISA-N |
| LogP | 3.83 |
| Synonyms |
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Applications:
UV-Vis Spectrum of Methyl Red
July 18, 2024
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Separation of Methyl Red and Ethyl Red on Primesep 100 Column
December 22, 2022
High Performance Liquid Chromatography (HPLC) Method for Separation of Methyl Red and Ethyl Red on Primesep 100 by SIELC Technologies.
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Separation of Меthyl Red and Ethyl Red
Methyl Red is a dark-red, single-charged basic dye that turns a deep red in acidic (low pH) solutions. Ethyl Red is a magenta-colored, single-charged basic dye. These two basic dyes can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the Visible spectrum at 520 nm.
Condition
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | Vis, 520 nm |
| Peak Retention Time | 6.19, 7.28 min |
Description
| Class of Compounds | Dyes |
| Analyzing Compounds | Меthyl red, Ethyl red |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsMethyl Red
HPLC Method for Analysis of Methyl red on Primesep 100 Column
December 22, 2022
HPLC Method for Analysis of Methyl Red on Primesep 100 by SIELC Technologies.
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Methyl red
Methyl red is a dark-red, single-charged basic dye that turns a deep red in acidic (low pH) solutions. This basic dye can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the Visible spectrum at 520 nm.
Condition
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 520 nm |
| Peak Retention Time | 6.19 min |
Description
| Class of Compounds | Dyes |
| Analyzing Compounds | Methyl Red |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
HPLC Method for Analysis of Dyes
March 1, 2018
Alizarin a red pigment that comes as a red powder with a slight orange tint. When dissolved it appears bright red with a slight pink undertone. Alizarin is also known as Mordant Red 11 or Turkey Red. Eosin Y is a pigment that comes as an orange powder that when dissolved in water turns bright orange. Eosin is a fluorescent staining agent. It is used to stain red blood cells, proteins on cytoplasm, collage, and muscle fibers. It is also fluorescence. Fluorescein is a dye that comes in a dark orange powder and is used as a tracer and an indicator. As it says in the name, it is also Fluorescent, obtaining a green-yellow color when under black light. Methyl red an indicator that turns red in acidic solutions (below 4.4 pH), is a shade of orange between 4.5 and 6.1 pH, and turns bright yellow in basic solutions (above pH 6.2). Nile blue, also known as Nile blue A, is used as a stain used in biology and histology as well as a pH indicator. In pH below 7 it becomes lighter shades of glue, and at pH 0 becomes almost completely translucent. At pH of seven it has a dark blue shade, while at pH higher than that it starts becoming more red. Pararosaniline Hydrochloride, also known as Basic Red 9, or C.I. 42500. It is a a magenta solid that is used as a dye. Patent Blue Violet, also known as Patent Blue, is a deep blue pigment that is often used to dye clothes. Resazurin is a blue dye as well as a pH indicator. At pH 3.8 or lower, it is bright orange, but the higher the pH the more blue it becomes, the color stops changing at the pH of 6.5 where it becomes indigo. Newcrom R1, a column that takes advantage of the newest technologies, does not contain embedded acidic nor basic ionizable groups and can retain dyes. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsAlizarin
Carmine
Eosin Y
Fluorescein
Fluorescein
Methyl Red
Nile Blue A
Pararosaniline Hydrochloride
Patent Blue Violet (Patent Blue)
Resazurin
HPLC Dye Separation on SHARC1 Column
July 10, 2013
| Column | Sharc 1, 3.2×100 mm, 3 µm, 100A |
| Mobile Phase | MeCN/EtOH – 99/1% |
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.5 ml/min |
| Detection | UV, 500, 650 nm |
| Class of Compounds |
Dye, Hydrophilic, Ionizable |
| Analyzing Compounds | Methyl Red, Methyl Orange, Methylene Blue, Nile Blue A |
Application Column
SHARC 1
The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.
Select optionsMethyl Red
Methylene Blue
Nile Blue A
