High Performance Liquid Chromatography (HPLC) Method for Separation of Methyl Red and Ethyl Red on Primesep 100 by SIELC Technologies.
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Separation of Меthyl Red and Ethyl Red
Methyl Red is a dark-red, single-charged basic dye that turns a deep red in acidic (low pH) solutions. Ethyl Red is a magenta-colored, single-charged basic dye. These two basic dyes can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the Visible spectrum at 520 nm.
Condition
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | Vis, 520 nm |
| Peak Retention Time | 6.19, 7.28 min |
Description
| Class of Compounds | Dyes |
| Analyzing Compounds | Меthyl red, Ethyl red |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsMethyl Red
