HPLC Method for Analysis of Methyl red on Primesep 100 Column

HPLC Method for Analysis of Methyl Red on Primesep 100 by SIELC Technologies.

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Меthyl Red on Primesep 100 Column
HPLC Method for Separation of Methyl Red on Primesep 100 Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Methyl red

Methyl red is a dark-red, single-charged basic dye that turns a deep red in acidic (low pH) solutions. This basic dye can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the Visible spectrum at 520 nm.

Condition

ColumnPrimesep 100, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O – 60/40%
BufferH3PO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV, 520 nm
Peak Retention Time6.19 min

Description

Class of CompoundsDyes
Analyzing CompoundsMethyl Red

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Methyl Red
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.