HPLC Method for Analysis of Methyl Red on Primesep 100 by SIELC Technologies.
Methyl Red, also known as C.I. Acid Red 2, is a dark-red, single-charged basic dye that turns a deep red in acidic (low pH) solutions and yellow at solution with pH above 6.2. It has the chemical formula C15H15N3O2. You can find detailed UV spectra of Methyl Red and information about its various lambda maxima by visiting the following link.
Methyl Red can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the Visible spectrum at 520 nm.
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 520 nm |
| Peak Retention Time | 6.19 min |
Description
| Class of Compounds | Dyes |
| Analyzing Compounds | Methyl Red |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
Methyl RedApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
