Chlorpyrifos-methyl (Dursban)

Chlorpyrifos-methyl structural formula

CAS Number5598-13-0
Molecular FormulaC7H7Cl3NO3PS
Molecular Weight322.520
InChI KeyHRBKVYFZANMGRE-UHFFFAOYSA-N
LogP4.31
Synonyms
  • Chlorpyrifos-methyl
  • O,O-Dimethyl O-(3,5,6-trichloropyridin-2-yl) phosphorothioate
  • Phosphorothioic acid, O,O-dimethyl O-(3,5,6-trichloro-2-pyridinyl) ester
  • 5598-13-0
  • Phosphorothioic acid, O,O-dimethylO-(3,5,6-trichloro-2-pyridinyl) ester
  • Chlorpyrifos M
  • Chlorpyriphos-methyl
  • clorpirifos-metil
  • Dursban ME
  • Dursban methyl
  • Methyl chlorpyrifos
  • Methyl Dursban
  • Noltran
  • O-(3,5,6-Trichloro-2-pyridinyl) O,O-dimethyl phosphorothioate
  • O,O-Dimethyl O-(3,5,6-trichloro-2-pyridyl) phosphorothioate
  • O,O-Dimethyl O-(3,5,6-trichloro-2-pyridyl) thiophosphate
  • O,O-Dimethyl-O-(3,5,6-trichloro-2-pyridyl)phosphorothioate
  • Phosphorothioic acid, O,O-dimethyl O-(3,5,6-trichloro-2-pyridinyl) ester
  • Phosphorothioic acid, O,O-dimethyl O-(3,5,6-trichloro-2-pyridyl) ester
  • BRN 1541078
  • Caswell No. 179AA
  • Chloropyriphos-methyl
  • Chlorpyrifos methyl
  • O,O-Dimethyl O-(3,5,6-trichloro-2-pyridinyl) phosphorothioate
  • O,O-Dimethyl O-3,5,6-trichloro-2-pyridyl phosphorothioate
  • O,O-Dimethyl O-(3,5,6-trichloro-2-pyridyl)phosphorothioate
  • EINECS 227-011-5
  • EPA Pesticide Chemical Code 059102
  • Methylchlorpyrifos
  • NSC 60380
  • Reldan 50 EC
  • Trichlormethylfos
  • Zertell
  • UNII-O49S38267J
  • Chlorpyrifos O,O-dimethyl analog
  • Methyl chlorpyriphos
  • O,O-dimethyl O-(3,5,6-trichloropyridin-2-yl) thiophosphate
  • Trichlormethylfos

Applications:

HPLC Separation of Pesticides on Newcrom B Column

February 3, 2020

Condition

Column Newcrom B, 4.6×150 mm, 3 µm, 100A
Mobile Phase MeCN/H2O
Buffer Formic Acid
Flow Rate 1.0 ml/min
Detection UV, 260, 270 nm

Description

Class of Compounds Hydrophobic, Herbicide, Pesticide
Analyzing Compounds Metalaxyl, Propoxur, Paclobutrazol, Abamectin, Propanil, Iprodione,  Dursban, Pentachlorophenol, Fenvalerate

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Abamectin
Chlorpyrifos-methyl (Dursban)
Fenvalerate
Iprodione
Metalaxyl
Paclobutrazol
Pentachlorophenol
Propanil
Propoxur
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of 12 Pesticides on Newcrom B Column

January 24, 2020


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Condition

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Formic Acid
Flow Rate 1.0 ml/min
Detection UV, 260 nm

Description

Class of Compounds Hydrophobic, Herbicide, Pesticide
Analyzing Compounds 6-Benzylaminopurine, Metalaxyl, Prochloraz, Paclobutrazol, Procymidone, Propanil, Chlorothalonil, Abamectin, Bifenazate, Dursban, Pentachlorophenol, Fenvalerate

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
6-Benzylaminopurine
Abamectin
Bifenazate
Chlorothalonil
Chlorpyrifos-methyl (Dursban)
Fenvalerate
Metalaxyl
Paclobutrazol
Pentachlorophenol
Prochloraz
Procymidone
Propanil
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of Dursban on Newcrom B Column

January 6, 2020


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HPLC Determination of Dursban on Newcrom B Column_1216

Condition

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 60/40%
Buffer AmFm pH 3.0 –  20 mM
Flow Rate 1.0 ml/min
Detection UV, 280 nm

Description

Class of Compounds Hydrophobic, Herbicide, Pesticide
Analyzing Compounds Chlorpyrifos-methyl (Dursban)

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Chlorpyrifos-methyl (Dursban)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Retention of Chlorpyrifos-methyl on Primesep 100 and Obelisc R Mixed-Mode Columns

September 14, 2015

 

Chlorpyrifos-methyl, an organophosphate pesticide is considered a target pesticide by the EURL (European Union Reference Laboratory) for the EUPT-FF9 2015 a proficiency test for the analysis of a over a hundred pesticides. This compound was separated on two different mixed-mode columns, Primesep 100 and Obelisc R. Primesep 100 is a reverse phase column that contains embedded acidic ion-pairing groups, while Obelisc R retains with long hydrophobic chains and multiple ionic pairing groups on the surface. Method is LC/MS compatible and can be replicated to analyze dozens of other pesticides.

Condition 1

Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 25-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm

 

Condition 2

Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm

 

Condition 3

Column Primesep 100, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Chlorpyrifos-methyl

 

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Chlorpyrifos-methyl (Dursban)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Chlorpyrifos-methyl Separation Using Obelisc and Primesep Columns

August 24, 2015

Chlorpyrifos and Chlorpyrifos-methyl are amongst the most widely used pesticides. Chlorpyrifos is considered a target pesticide by the EURL (European Union Reference Laboratory) for the EURL-CF9 and Obelisc R and Primesep B2 were used to separate chlorpyrifos-methyl and its impurities using a single multiresidue method, which can be used for many pesticides. Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations, while Primesep B2 contains embedded basic ion-pairing groups. Method is LC/MS compatible and can be used as a general approach for analyzing organophosphate pesticides.

Condition 1

Column Primesep B2, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 4,5- 20-50 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm

 

Condition 2

Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 4,5- 20-50 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm

 

 

Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Chlorpyrifos-methyl

 

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options

Primesep B2

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options

Primesep C

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Chlorpyrifos-methyl (Dursban)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Analysis of Chlorpyrifos-methyl

August 10, 2015

Chlorpyrifos-methyl is an organiphosphate insecticide also known and Dursban and Lorsban. It has been banned for home use since 2001 due to its toxicity, but is one of the most widely used organophosphate insecticides according to the EPA. Chlorpyrifos-methyl is on the EURL–SRM list of target pesticides in cereals and feeding stuffs. Chlorpyrifos-methyl was retained on Obelisc R, a mixed-mode HPLC column which contains ionic groups and hydrophobic chain. Method is LC/MS compatible and can be used as a general approach for analyzing chlorpyrifos-methyl and other organophosphate insecticides.

Condition 

Column Obelisc R, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 30%
Buffer AmFm pH 3.0- 20 mM
Flow Rate 1.0 ml/min
Detection UV, 250 nm

Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Chlorpyrifos-methyl

 

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Chlorpyrifos-methyl (Dursban)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.