HPLC Method for 6-Benzylaminopurine on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of 6-Benzylaminopurine

6-Benzylaminopurine is a plant growth regulation compound with the molecular formula C₁₂H₁₁N₅. It’s presence in asparagus, for example, leads to deeper color, increased firmness, and a decrease in fibrous hardness. In general, it stimulates cell division and differentiation.

6-Benzylaminopurine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

HPLC Method for 6-Benzylaminopurine on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of 6-Benzylaminopurine

6-Benzylaminopurine is a plant growth regulation compound with the molecular formula C₁₂H₁₁N₅. It’s presence in asparagus, for example, leads to deeper color, increased firmness, and a decrease in fibrous hardness. In general, it stimulates cell division and differentiation.

6-Benzylaminopurine can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

High Performance Liquid Chromatography (HPLC) MS Method for Analysis of trans-Zeatin, Kinetin, N6-(Δ2-Isopentenyl)adenine, Thidiazuron, 6-Benzylaminopurine, Brassinazole on Primesep 200 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of trans-Zeatin, Kinetin, N6-(Δ2-Isopentenyl)adenine, Thidiazuron, 6-Benzylaminopurine, Brassinazole

It looks like you’re listing a series of compounds, most of which are cytokinins or related plant hormones. Here’s a brief overview and classification for the compounds you mentioned:

trans-Zeatin:

• A type of cytokinin.
• Naturally occurring in plants and plays a key role in promoting cell division and growth.

Kinetin:

• A synthetic cytokinin.
• Used in plant tissue culture to stimulate cell division.

N6-(Δ2-Isopentenyl)adenine (iP):

• Another cytokinin.
• Found in plants and is involved in regulating growth, development, and differentiation.

N6-Benzyladenine (BA):

• A synthetic cytokinin.
• Commonly used in agricultural practices to enhance plant growth and delay senescence.

Brassinazole:

• Not a cytokinin, but a brassinosteroid biosynthesis inhibitor.
• Used to study the role of brassinosteroids (another class of phytohormones) in plants.

Thidiazuron (TDZ):

• A phenylurea derivative that acts as a cytokinin-like compound.
• Widely used in plant tissue culture to promote shoot regeneration and induce cytokinin-like effects.

trans-Zeatin, Kinetin, N6-(Δ2-Isopentenyl)adenine, Thidiazuron, 6-Benzylaminopurine, Brassinazole can be retained, separated and analyzed using a Primesep 200 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and formic acid as a buffer. This method allows for detection using UV 290, 265 nm.

You can find detailed UV spectra of trans-Zeatin, Kinetin, N6-(Δ2-Isopentenyl)adenine, Thidiazuron, 6-Benzylaminopurine, Brassinazole and information about its various lambda maxima by visiting the following link.

High Performance Liquid Chromatography (HPLC) MS Method for Analysis of 6-Benzylaminopurine (N6-Benzyladenine) on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of 6-Benzylaminopurine

6-Benzylaminopurine (N6-Benzyladenine, 6-BAP or BAP) is a synthetic cytokinin commonly used in plant biology and tissue culture to stimulate cell division and growth. It is a potent growth regulator and is applied to enhance plant tissue differentiation, particularly for shoot induction.

Key properties:

• Function: Promotes cell division (cytokinesis), shoot formation, and delays senescence in plant tissues.
• Applications:
• Extensively used in plant tissue culture for micropropagation, promoting the growth of shoots in various plant species.
• Also used to improve fruit set and increase crop yields in agricultural practices.

6-Benzylaminopurine is one of the most commonly used cytokinins in horticulture and laboratory studies.

6-Benzylaminopurine can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 210 nm.

You can find detailed UV spectra of 6-Benzylaminopurine and information about its various lambda maxima by visiting the following link.

Condition

Description

HPLC Method for 6-Benzylaminopurine, Metalaxyl (UV), Prochloraz, Paclobutrazol, Propanil, Chlorothalonil, Chlorpyrifos-methyl (Dursban), Pentachlorophenol, Fenvalerate, Abamectin, Procymidone, Bifenazate on Newcrom B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of 6-Benzylaminopurine, Metalaxyl (UV), Prochloraz, Paclobutrazol, Propanil, Chlorothalonil, Chlorpyrifos-methyl (Dursban), Pentachlorophenol, Fenvalerate, Abamectin, Procymidone, Bifenazate.

6-Benzylaminopurine is a plant growth regulation compound with the molecular formula C₁₂H₁₁N₅. It’s presence in asparagus, for example, leads to deeper color, increased firmness, and a decrease in fibrous hardness. In general, it stimulates cell division and differentiation.

Metalaxyl, also known as methyl N-(methoxyacetyl)-N-(2,6-xylyl)-DL-alaninate,  is a systemic fungicide with the chemical formula C₁₅H₂₁NO₄.  It is used to control Pythium and Phytophthora in vegetables. It has suffered a resistance problem going back to the 1980s.

Prochloraz is an imidazole fungicide with the chemical formula C₁₅H₁₆Cl₃N₃O₂. It is used widely across Europe, Asia, South America, and Australia, but is not registered for use in the United States. It works as an inhibitor of the enzyme lanosterol 14α-demethylase.

Paclobutrazol (PBZ) is a synthetic triazole compound with the chemical formula C₁₅H₂₀ClN₃O. It is used to control plant growth as an antagonist of the plant hormone gibberellin. Through reducing reducing internodal growth, it causes stronger stems, greater root growth, and earlier fruitset. It can also reduce frost sensitivity in plants.

Procymidone is a pesticide and fungicide with the chemical formula  C₁₃H₁₁Cl₂NO₂. It is typically used to kill unwanted ferns, nettles, and fungi in lupins, grapes, stone fruit, and strawberries.

Propanil is a contact herbicide with the chemical formula C₉H₉Cl₂NO. It is one of the most widely used herbicides in America. It works as an inhibitor of photosynthesis and CO₂ fixation in weeds.

Chlorothalonil is a compound with a variety of uses as a fungicide, wood protectant, pesticide, and acaricide. It is used predominantly on peanuts, potatoes, and tomatoes. Outside of agriculture, it is also used in paints, resins, emulsions, and coatings. It’s chemical formula is C₈Cl₄N₂.

Abamectin is a insecticide with the chemical formulas  C48H72O14 and C47H70O14. It is also used to control fire ant populations. In veterinary cases, it is used to deworm horses as well as an antihelmintic.

Bifenazate is pesticide with the chemical formula C₁₇H₂₀N₂O₃. It is typically used to control a large variety of mite pests in plants in various settings. It works through modulating GABA receptors and inhibiting complex III of the mitochondrial electron transport chain.

Dursban is a highly toxic organophosphate with the chemical formula C₉H₁₁Cl₃NO₃PS. It works through interrupting the electrochemical processes in nerves.  This leads to a build-up of acetylcholine, which leads to paralysis and eventual death.

Pentachlorophenol is a manufactured chemical with the chemical formula C₆HCl₅O. It is most often used as herbicide, insecticide, fungicide, algaecide, and disinfectant. Exposure to it can cause damage to liver, kidney, blood, lungs, eyes, skin, and mouth. It is classified as a probable human carcinogen.

Fenvalerate is synthetic pyrethroid insecticide with the chemical formula C₂₅H₂₂ClNO₃. It is used against a wide range of pests, partially due to it’s moderate mammalian toxicity following short-term and acute exposure. It is the most toxic to bees and fish.  Upon contact, it may irritate skin and eyes.

6-Benzylaminopurine, Metalaxyl (UV), Prochloraz, Paclobutrazol, Propanil, Chlorothalonil, Chlorpyrifos-methyl (Dursban), Pentachlorophenol, Fenvalerate, Abamectin, Procymidone, Bifenazate can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using UV.

HPLC Method for 6-Benzylaminopurine on Newcrom B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of 6-Benzylaminopurine.

6-Benzylaminopurine is a plant growth regulation compound with the molecular formula C₁₂H₁₁N₅. It’s presence in asparagus, for example, leads to deeper color, increased firmness, and a decrease in fibrous hardness. In general, it stimulates cell division and differentiation.

6-Benzylaminopurine can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate[ buffer. Detection is performed using UV.