HPLC Determination of 6-Benzylaminopurine on Newcrom R1 Column

HPLC Method for 6-Benzylaminopurine on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of 6-Benzylaminopurine.

6-Benzylaminopurine is a plant growth regulation compound with the molecular formula C12H11N5. It’s presence in asparagus, for example, leads to deeper color, increased firmness, and a decrease in fibrous hardness. In general, it stimulates cell division and differentiation. In floristry it is used to extend the shelf life of cut flowers and greens. You can find detailed UV spectra of 6-Benzylaminopurine and information about its various lambda maxima by visiting the following link.

6-Benzylaminopurine can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.

Condition

ColumnNewcrom R1, 2.1 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 20/80%
BufferAmFm pH 3.0 –  20 mM
Flow Rate0.2 ml/min
DetectionUV, 272 nm

Description

Class of CompoundsHydrophobic, Herbicide, Pesticide
Analyzing Compounds6-Benzylaminopurine

Application Column

Newcrom R1

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
6-Benzylaminopurine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.