Brassinazole

High Performance Liquid Chromatography (HPLC) MS Method for Analysis of trans-Zeatin, Kinetin, N6-(Δ2-Isopentenyl)adenine, Thidiazuron, 6-Benzylaminopurine, Brassinazole on Primesep 200 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of trans-Zeatin, Kinetin, N6-(Δ2-Isopentenyl)adenine, Thidiazuron, 6-Benzylaminopurine, Brassinazole

It looks like you’re listing a series of compounds, most of which are cytokinins or related plant hormones. Here’s a brief overview and classification for the compounds you mentioned:

trans-Zeatin:

• A type of cytokinin.
• Naturally occurring in plants and plays a key role in promoting cell division and growth.

Kinetin:

• A synthetic cytokinin.
• Used in plant tissue culture to stimulate cell division.

N6-(Δ2-Isopentenyl)adenine (iP):

• Another cytokinin.
• Found in plants and is involved in regulating growth, development, and differentiation.

N6-Benzyladenine (BA):

• A synthetic cytokinin.
• Commonly used in agricultural practices to enhance plant growth and delay senescence.

Brassinazole:

• Not a cytokinin, but a brassinosteroid biosynthesis inhibitor.
• Used to study the role of brassinosteroids (another class of phytohormones) in plants.

Thidiazuron (TDZ):

• A phenylurea derivative that acts as a cytokinin-like compound.
• Widely used in plant tissue culture to promote shoot regeneration and induce cytokinin-like effects.

trans-Zeatin, Kinetin, N6-(Δ2-Isopentenyl)adenine, Thidiazuron, 6-Benzylaminopurine, Brassinazole can be retained, separated and analyzed using a Primesep 200 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and formic acid as a buffer. This method allows for detection using UV 290, 265 nm.

You can find detailed UV spectra of trans-Zeatin, Kinetin, N6-(Δ2-Isopentenyl)adenine, Thidiazuron, 6-Benzylaminopurine, Brassinazole and information about its various lambda maxima by visiting the following link.