| CAS Number | 1214-39-7 |
|---|---|
| Molecular Formula | C12H11N5 |
| Molecular Weight | 225.25 |
| InChI Key | NWBJYWHLCVSVIJ-UHFFFAOYSA-N |
| LogP | 2.1 |
| Synonyms |
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Applications:
HPLC MS Method for the Analysis of Cytokinins: A Phytohormone Class and Brassinazole on Primesep 200 Column
September 11, 2024
High Performance Liquid Chromatography (HPLC) MS Method for Analysis of trans-Zeatin, Kinetin, N6-(Δ2-Isopentenyl)adenine, Thidiazuron, 6-Benzylaminopurine, Brassinazole on Primesep 200 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of trans-Zeatin, Kinetin, N6-(Δ2-Isopentenyl)adenine, Thidiazuron, 6-Benzylaminopurine, Brassinazole
It looks like you’re listing a series of compounds, most of which are cytokinins or related plant hormones. Here’s a brief overview and classification for the compounds you mentioned:
trans-Zeatin:
- A type of cytokinin.
- Naturally occurring in plants and plays a key role in promoting cell division and growth.
Kinetin:
- A synthetic cytokinin.
- Used in plant tissue culture to stimulate cell division.
N6-(Δ2-Isopentenyl)adenine (iP):
- Another cytokinin.
- Found in plants and is involved in regulating growth, development, and differentiation.
N6-Benzyladenine (BA):
- A synthetic cytokinin.
- Commonly used in agricultural practices to enhance plant growth and delay senescence.
Brassinazole:
- Not a cytokinin, but a brassinosteroid biosynthesis inhibitor.
- Used to study the role of brassinosteroids (another class of phytohormones) in plants.
Thidiazuron (TDZ):
- A phenylurea derivative that acts as a cytokinin-like compound.
- Widely used in plant tissue culture to promote shoot regeneration and induce cytokinin-like effects.
trans-Zeatin, Kinetin, N6-(Δ2-Isopentenyl)adenine, Thidiazuron, 6-Benzylaminopurine, Brassinazole can be retained, separated and analyzed using a Primesep 200 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and formic acid as a buffer. This method allows for detection using UV 290, 265 nm.
You can find detailed UV spectra of trans-Zeatin, Kinetin, N6-(Δ2-Isopentenyl)adenine, Thidiazuron, 6-Benzylaminopurine, Brassinazole and information about its various lambda maxima by visiting the following link.
| Column | Primesep 200, 2.1 x 100 mm, 5 µm, 100 A |
| Mobile Phase | MeCN – 20%, 4 min than Gradient MeCN/H2O – 20-90% 16 min |
| Buffer | Formic Acid -0.2% |
| Flow Rate | 0.2 mg/ml |
| Detection | UV 290 nm, 265 nm ESI SIM 220+, 216+, 204+,226+, 328+, 221+ |
| Class of Compounds | Phytohormone |
| Analyzing Compounds | trans-Zeatin, Kinetin, N6-(Δ2-Isopentenyl)adenine, Thidiazuron, 6-Benzylaminopurine, Brassinazole |
Application Column
Primesep 200
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Brassinazole
Kinetin
N6-(Δ2-Isopentenyl)adenine
Thidiazuron
trans-Zeatin
LC MS Detection
HPLC Method for Analysis of 6-Benzylaminopurine on Primesep 100 Column
September 11, 2024
High Performance Liquid Chromatography (HPLC) MS Method for Analysis of 6-Benzylaminopurine on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of 6-Benzylaminopurine
6-Benzylaminopurine (6-BAP or BAP) is a synthetic cytokinin commonly used in plant biology and tissue culture to stimulate cell division and growth. It is a potent growth regulator and is applied to enhance plant tissue differentiation, particularly for shoot induction.
Key properties:
- Function: Promotes cell division (cytokinesis), shoot formation, and delays senescence in plant tissues.
- Applications:
- Extensively used in plant tissue culture for micropropagation, promoting the growth of shoots in various plant species.
- Also used to improve fruit set and increase crop yields in agricultural practices.
6-Benzylaminopurine is one of the most commonly used cytokinins in horticulture and laboratory studies.
6-Benzylaminopurine can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 210 nm.
You can find detailed UV spectra of 6-Benzylaminopurine and information about its various lambda maxima by visiting the following link.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN – 50% |
| Buffer | H2SO4 -0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 205 nm |
| Sample | 0.1 mg/ml |
| Diluent | MeCN/H2O- 50/50% |
| LOD* | 5 ppb |
| Class of Compounds | Phytohormone |
| Analyzing Compounds | 6-Benzylaminopurine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
HPLC Determination of 6-Benzylaminopurine on Newcrom R1 Column
June 11, 2024
Condition
| Column | Newcrom R1, 2.1 x 100 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | AmFm pH 3.0 – 20 mM |
| Flow Rate | 0.2 ml/min |
| Detection | UV, 272 nm |
Description
| Class of Compounds | Hydrophobic, Herbicide, Pesticide |
| Analyzing Compounds | 6-Benzylaminopurine |
Application Column
Newcrom R1
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
HPLC Separation of 12 Pesticides on Newcrom B Column
January 24, 2020
| Column | Newcrom B, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | Formic Acid |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 260 nm |
| Class of Compounds | Hydrophobic, Herbicide, Pesticide |
| Analyzing Compounds | 6-Benzylaminopurine, Metalaxyl, Prochloraz, Paclobutrazol, Procymidone, Propanil, Chlorothalonil, Abamectin, Bifenazate, Dursban, Pentachlorophenol, Fenvalerate |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsAbamectin
Bifenazate
Chlorothalonil
Chlorpyrifos-methyl (Dursban)
Fenvalerate
Metalaxyl (UV)
Paclobutrazol
Pentachlorophenol
Prochloraz
Procymidone
Propanil
HPLC Determination of 6-Benzylaminopurine on Newcrom B Column
January 10, 2020
| Column | Newcrom B, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | AmFm pH 3.0 – 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 272 nm |
| Class of Compounds | Hydrophobic, Herbicide, Pesticide |
| Analyzing Compounds | 6-Benzylaminopurine |
Application Column
Newcrom B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
