Chlorpyrifos-methyl Separation Using Obelisc and Primesep Columns

Chlorpyrifos and Chlorpyrifos-methyl are amongst the most widely used pesticides. Chlorpyrifos is considered a target pesticide by the EURL (European Union Reference Laboratory) for the EURL-CF9 and Obelisc R and Primesep B2 were used to separate chlorpyrifos-methyl and its impurities using a single multiresidue method, which can be used for many pesticides. Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations, while Primesep B2 contains embedded basic ion-pairing groups. Method is LC/MS compatible and can be used as a general approach for analyzing organophosphate pesticides.

Condition 1

Column Primesep B2, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 4,5- 20-50 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 4,5- 20-50 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm
   

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Chlorpyrifos-methyl
 
SIELC Technologies usually develops more than one method for each compound on our website. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound of interest.
Application Analytes:
Chlorpyrifos-methyl (Dursban)

Application Detection:
UV Detection