HPLC Analysis of Chlorpyrifos-methyl

Chlorpyrifos-methyl is an organiphosphate insecticide also known and Dursban and Lorsban. It has been banned for home use since 2001 due to its toxicity, but is one of the most widely used organophosphate insecticides according to the EPA. Chlorpyrifos-methyl is on the EURL–SRM list of target pesticides in cereals and feeding stuffs. Chlorpyrifos-methyl was retained on Obelisc R, a mixed-mode HPLC column which contains ionic groups and hydrophobic chain. Method is LC/MS compatible and can be used as a general approach for analyzing chlorpyrifos-methyl and other organophosphate insecticides.


Column Obelisc R, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 30%
Buffer AmFm pH 3.0- 20 mM
Flow Rate 1.0 ml/min
Detection UV, 250 nm


Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Chlorpyrifos-methyl
Application Analytes:
Chlorpyrifos-methyl (Dursban)