HPLC Analysis of Chlorpyrifos-methyl

Chlorpyrifos-methyl is an organiphosphate insecticide also known and Dursban and Lorsban. It has been banned for home use since 2001 due to its toxicity, but is one of the most widely used organophosphate insecticides according to the EPA. Chlorpyrifos-methyl is on the EURL–SRM list of target pesticides in cereals and feeding stuffs. Chlorpyrifos-methyl was retained on Obelisc R, a mixed-mode HPLC column which contains ionic groups and hydrophobic chain. Method is LC/MS compatible and can be used as a general approach for analyzing chlorpyrifos-methyl and other organophosphate insecticides.

Condition 
Column Obelisc R, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 30%
Buffer AmFm pH 3.0- 20 mM
Flow Rate 1.0 ml/min
Detection UV, 250 nm

Description
Class of Compounds
  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Chlorpyrifos-methyl

 

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Chlorpyrifos-methyl (Dursban)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.