Ionic Modifier Effect on Selectivity of Separation of Deoxyguanosine, Guanine and Guanosine on BIST B+ by SIELC Technologies.

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analyses of Deoxyguanosine, Guanine and Guanosine

Description

HPLC Method for Analysis of Deoxyguanosine, Guanine and Guanosine on BIST B+ by SIELC Technologies.

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analyses of Deoxyguanosine, Guanine and Guanosine

Description

HPLC Method for Deoxyguanosine, 2'-Deoxy-2'-fluoroguanosine on Newcrom AH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of 2′-Deoxy-2′-fluoroguanosine and Deoxyguanosine

Nucleosides are the building blocks for DNA and RNA as well as other roles in biomechanical processes such as signal transduction. 2′-Deoxy-2′-fluoroguanosine is an antiviral nuceloside that can help the body fight influenza A & B. Deoxyguanosine is one of the four core deoxyribonucleosides that compose DNA. The only difference chemically is the addition of a fluorine atom on the non-Nitrogen ring.

2′-Deoxy-2′-fluoroguanosine and Deoxyguanosine can be detected in the high UV regime. Using a Newcrom AH mixed-mode column and a mobile phase consisting of just water (without a buffer), 2′-Deoxy-2′-fluoroguanosine and Deoxyguanosine can be retained, separated, and analyzed. This analysis method can be UV detected at 252 nm with high resolution. This method is compatible with Mass Spectrometry.

HPLC Method for Adenosine, Guanosine, Deoxyguanosine, Deoxyadenosine on Newcrom AH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Adenosine, Guanosine, Deoxyguanosine, Deoxyadenosine.

Guanosine is a purine nucleoside with the chemical formula C₁₀H₁₃N₅O₅. It can be phosphorylated into many other forms, which play vital roles in biochemical possesses like synthesis of nucleic acids, proteins, photosynthesis, and more. It is also required for RNA splicing.

Deoxyguanosine is a deoxyribonucleoside with the chemical formula C₁₀H₁₃N₅O₄. It is a vital part of what makes up DNA.

Adenosine is a key building block of energy-carrying molecules with the chemical formula C₁₀H₁₃N₅O₄. It has a variety of other uses, including being a inhibitory neurotransmitter which helps with sleep and acting as a blood flow regulator. Medicinally, it is used as treatment for supraventricular tachycardia (SVT). You can find detailed UV spectra of Adenosine and information about its various lambda maxima by visiting the following link.

Deoxyadenosine is a deoxyribonucleoside with the chemical formula C₁₀H₁₃N₅O₃. It is a derivative of adenosine. High presence of it can kill T lymphocytes and kill those cells, leading to adenosine deaminase severe combined immunodeficiency disease, also known as ADA-SCID.

Adenosine, Guanosine, Deoxyguanosine, Deoxyadenosine are the building blocks for DNA and RNA as well as other roles in biomechanical processes such as signal transduction. By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, nucleosides: guanosine, deoxyguanosine, adenosine, and deoxyadenosine, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and H3PO4 as a buffer. UV detection at 210 nm.

Primesep 200 separates with baseline resolution the nucleosides (deoxyuridine, deoxyguanosine, deoxycytidine, deoxyadenozine) by a combination of cation exchange and reversed phase. These compounds are not retained on a traditional C18 column. Primesep 200 has an embedded anionic functional group which helps retain polar compounds by polar and ion-exchange mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 270 nm.