CAS Number | 40627-14-3 |
---|---|
Molecular Formula | C10H13N5O3 |
Molecular Weight | 251.246 |
InChI Key | OLXZPDWKRNYJJZ-RRKCRQDMSA-N |
LogP | -0.5 |
Synonyms |
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Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
Column | BIST B+, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN – 85% |
Buffer | H3PO4 – 0.2% |
Flow Rate | 1.0 ml/min |
Detection | UV 260 nm |
Peak Retention Time | 8.2, 9.8 min |
Class of Compounds | Nucleosides |
Analyzing Compounds | 3′-Deoxyadenosine (Cordycepin) and 2′-Deoxyadenosine (Deoxyadenosine) |
SIELC Technologies’ BIST™ Columns are a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns. When used with our BIST™ mobile phases, these ion exchange columns can generate very strong retention of analytes that have the same charge polarity as the stationary phase, unlocking new chromatography applications that were previously too difficult to achieve.
Select optionsSeparation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
Column | BIST B+, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN – 85% |
Buffer | H2SO4 – 0.2% |
Flow Rate | 1.0 ml/min |
Detection | UV 260 nm |
Peak Retention Time | 2.8, 3.2, 4.3 min |
Class of Compounds | Nucleosides |
Analyzing Compounds | Adenine, Deoxyadenosine and Adenosine |
SIELC Technologies’ BIST™ Columns are a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns. When used with our BIST™ mobile phases, these ion exchange columns can generate very strong retention of analytes that have the same charge polarity as the stationary phase, unlocking new chromatography applications that were previously too difficult to achieve.
Select optionsSeparation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method of Adenosine and Deoxyadenosine.
Nucleosides are the building blocks for DNA and RNA as well as other roles in biomechanical processes such as signal transduction. By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, nucleosides: adenosine and deoxyadenosine, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and ammonium formate (AmFm) buffer. Detection can be achieved with UV 260 nm, mass spectrometry (MS), evaporative light scattering detection (ELSD) and Charged aerosol detection (,.CAD).
Column | Newcrom AH, 3.2×100 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 10/90% |
Buffer | AmFm pH 3.0 – 10 mM |
Flow Rate | 1.0 ml/min |
Detection | UV, 260 nm |
Class of Compounds |
Nucleatide |
Analyzing Compounds | Adenosine, Deoxyadenosine |
The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse phase column with low silanol activity.
Select optionsSeparation type: Liquid Chromatography Mixed-mode
Nucleosides are the building blocks for DNA and RNA as well as other roles in biomechanical processes such as signal transduction. By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, nucleosides: guanosine, deoxyguanosine, adenosine, and deoxyadenosine, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and H3PO4 as a buffer. UV detection at 210 nm.
Column | Newcrom AH, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 20/80% |
Buffer | H3PO4 – 0.5% |
Flow Rate | 1.0 ml/min |
Detection | UV, 210 nm |
Class of Compounds |
Nucleoside, Hydrophilic, Ionizable |
Analyzing Compounds | Guanosine, Deoxyguanosine, Adenosine, Deoxyadenosine |
The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse phase column with low silanol activity.
Select optionsColumn | Sharc 1, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN/MeOH |
Buffer | AmFm, Formic acid |
Flow Rate | 1.0 ml/min |
Detection | UV, 270 nm |
Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements |
Analyzing Compounds | Thymidine, Uridine, Deoxyadenosine, Adenosine, Deoxyguanosine, Guanosine, Deoxycytidine, Cytidine |
The SHARC™ family of innovative columns are are the first commercially available columns with separation based primarily on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding is an interaction (attraction) of bound hydrogen atom to the molecules with electronegative atoms such as oxygen, nitrogen, and fluorine.
Select optionsNucleosides consisting of deoxyribose and a nucleobase cannot be separated on a common C18 column. By using a Primesep 200 mixed-mode column with a cation-exchange mechanism, nucleosides: deoxyuridine, deoxyguanosine, deoxycytidine and deoxyadenosine, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN) and TFA as buffer. UV detection at 270nm.
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select optionsPrimesep 200 separates with baseline resolution the nucleosides (deoxyuridine, deoxyguanosine, deoxycytidine, deoxyadenozine) by a combination of cation exchange and reversed phase. These compounds are not retained on a traditional C18 column. Primesep 200 has an embedded anionic functional group which helps retain polar compounds by polar and ion-exchange mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 270 nm.
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
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