Deoxyadenosine

Deoxyadenozine

CAS Number40627-14-3
Molecular FormulaC10H13N5O3
Molecular Weight251.246
InChI KeyOLXZPDWKRNYJJZ-RRKCRQDMSA-N
LogP-0.5
Synonyms
  • 2'-deoxyadenosine
  • deoxyadenosine
  • 2-Deoxyadenosine
  • (2R,3S,5R)-5-(6-amino-9H-purin-9-yl)-2-(hydroxymethyl)tetrahydrofuran-3-ol
  • Adenyldeoxyriboside
  • Adenine deoxyribonucleoside
  • Adenine deoxyribose
  • Desoxyadenosine
  • CCRIS 1782
  • UNII-P582C98ULC
  • 2'-dA
  • Adenine deoxyriboside
  • Adenine deoxy nucleoside
  • CHEBI:17256
  • 2'-deoxy-d-adenosine
  • AI3-52383
  • ADENOSINE, 2'-DEOXY-
  • (2R,3S,5R)-5-(6-amino-9H-purin-9-yl)-2-(hydroxymethyl)oxolan-3-ol
  • EINECS 213-488-7

Applications:

HPLC Method for Analysis of 3′-Deoxyadenosine and 2′-Deoxyadenosine on BIST B+ Column

2022-12-01

HPLC Method for Analysis of 3′-Deoxyadenosine (Cordycepin) and 2′-Deoxyadenosine (Deoxyadenosine) on BIST B+ by SIELC Technologies.

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of 3′-Deoxyadenosine and 2′-Deoxyadenosine on BIST B+ Column
HPLC Method for Analysis of 3′-Deoxyadenosine (Cordycepin) and 2′-Deoxyadenosine (Deoxyadenosine) on BIST B+ by SIELC Technologies.

High Performance Liquid Chromatography (HPLC) Method for Analyses of 3′-Deoxyadenosine (Cordycepin) and 2′-Deoxyadenosine (Deoxyadenosine)


Condition

ColumnBIST B+, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN – 85%
BufferH3PO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 260 nm
Peak Retention Time8.2, 9.8 min

Description

Class of CompoundsNucleosides
Analyzing Compounds3′-Deoxyadenosine (Cordycepin) and 2′-Deoxyadenosine (Deoxyadenosine)

Application Column

BIST B+

SIELC Technologies’ BIST™ Columns are a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns. When used with our BIST™ mobile phases, these ion exchange columns can generate very strong retention of analytes that have the same charge polarity as the stationary phase, unlocking new chromatography applications that were previously too difficult to achieve.

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Application Analytes:
Cordycepin
Deoxyadenosine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Separation of Adenine, Deoxyadenosine and Adenosine on BIST B+ Column

2022-11-28

HPLC Method for Separation of Adenine, Deoxyadenosine and Adenosine on BIST B+ by SIELC Technologies.

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Separation of Adenine, Deoxyadenosine and Adenosine on BIST B+ Column
HPLC Method for Separation of Adenine, Deoxyadenosine and Adenosine on BIST B+ by SIELC Technologies.

Condition

ColumnBIST B+, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN – 85%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 260 nm
Peak Retention Time2.8, 3.2, 4.3 min

Description

Class of CompoundsNucleosides
Analyzing CompoundsAdenine, Deoxyadenosine and Adenosine

Application Column

BIST B+

SIELC Technologies’ BIST™ Columns are a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns. When used with our BIST™ mobile phases, these ion exchange columns can generate very strong retention of analytes that have the same charge polarity as the stationary phase, unlocking new chromatography applications that were previously too difficult to achieve.

Select options
Application Analytes:
Adenine
Adenosine
Deoxyadenosine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Adenosine and Deoxyadenosine on Newcrom AH Column

2021-05-26

Separation type: Liquid Chromatography Mixed-mode


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High Performance Liquid Chromatography (HPLC) Method of Adenosine and Deoxyadenosine.

Nucleosides are the building blocks for DNA and RNA as well as other roles in biomechanical processes such as signal transduction. By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, nucleosides: adenosine and deoxyadenosine, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and ammonium formate (AmFm) buffer. Detection can be achieved with UV 260 nm, mass spectrometry (MS), evaporative light scattering detection (ELSD) and Charged aerosol detection (,.CAD).

Condition

Column Newcrom AH, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer  AmFm pH 3.0 – 10 mM
Flow Rate 1.0 ml/min
Detection UV, 260 nm

 

Description

Class of Compounds
Nucleatide
Analyzing Compounds Adenosine,  Deoxyadenosine

 

Application Column

Newcrom AH

The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse phase column with low silanol activity.

Select options
Application Analytes:
Adenosine
Deoxyadenosine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Guanosine, Deoxyguanosine, Adenosine, Deoxyadenosine on Newcrom AH Column

2021-05-25

Separation type: Liquid Chromatography Mixed-mode


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Nucleosides are the building blocks for DNA and RNA as well as other roles in biomechanical processes such as signal transduction. By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, nucleosides: guanosine, deoxyguanosine, adenosine, and deoxyadenosine, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and H3PO4 as a buffer. UV detection at 210 nm.

Condition

Column Newcrom AH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 20/80%
Buffer H3PO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Nucleoside,  Hydrophilic, Ionizable
Analyzing Compounds Guanosine, Deoxyguanosine, Adenosine, Deoxyadenosine

 

Application Column

Newcrom AH

The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse phase column with low silanol activity.

Select options
Application Analytes:
Adenosine
Deoxyadenosine
Deoxyguanosine
Guanosine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Nucleosides and Deoxynucleosides

2012-07-23

Condition

Column Sharc 1, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH
Buffer AmFm, Formic acid
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Thymidine, Uridine, Deoxyadenosine, Adenosine, Deoxyguanosine, Guanosine, Deoxycytidine, Cytidine

 

Application Column

SHARC 1

The SHARC™ family of innovative columns are are the first commercially available columns with separation based primarily on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding is an interaction (attraction) of bound hydrogen atom to the molecules with electronegative atoms such as oxygen, nitrogen, and fluorine.

Select options
Application Analytes:
Adenosine
Cytidine
Deoxyadenosine
Deoxycytidine
Deoxyguanosine
Guanosine
Thymidine
Uridine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Nucleobases (2)

2007-12-06


Nucleosides consisting of deoxyribose and a nucleobase cannot be separated on a common C18 column. By using a Primesep 200 mixed-mode column with a cation-exchange mechanism, nucleosides: deoxyuridine, deoxyguanosine, deoxycytidine and deoxyadenosine, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN) and TFA as buffer. UV detection at 270nm.

Application Column

Primesep 200

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

Select options
Application Analytes:
Deoxyadenosine
Deoxycytidine
Deoxyguanosine
Deoxyuridine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Deoxyribo Nucleic Acid

2003-09-24

Primesep 200 separates with baseline resolution the nucleosides (deoxyuridine, deoxyguanosine, deoxycytidine, deoxyadenozine) by a combination of cation exchange and reversed phase. These compounds are not retained on a traditional C18 column. Primesep 200 has an embedded anionic functional group which helps retain polar compounds by polar and ion-exchange mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 270 nm.

Application Column

Primesep 200

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

Select options
Application Analytes:

Deoxyadenosine
Deoxycytidine
Deoxyuridine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.