Separation type: Liquid Chromatography Mixed-mode View on hplc.cloud Nucleosides are the building blocks for DNA and RNA as well as other roles in biomechanical processes such as signal transduction. By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, nucleosides: guanosine, deoxyguanosine, adenosine, and deoxyadenosine, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and H3PO4 as a buffer. UV detection at 210 nm.
|Column||Newcrom AH, 4.6x150 mm, 5 µm, 100A|
|Mobile Phase||MeCN/H2O - 20/80%|
|Buffer||H3PO4 - 0.5%|
|Flow Rate||1.0 ml/min|
|Detection||UV, 210 nm|
|Class of Compounds||Nucleoside, Hydrophilic, Ionizable|
|Analyzing Compounds||Guanosine, Deoxyguanosine, Adenosine, Deoxyadenosine|