CAS Number | 118-00-3 |
---|---|
Molecular Formula | C10H13N5O5 |
Molecular Weight | 283.244 |
InChI Key | NYHBQMYGNKIUIF-UUOKFMHZSA-N |
LogP | -2.7 |
Synonyms |
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Applications:
Ionic Modifier Effect on Selectivity of Separation of Deoxyguanosine, Guanine and Guanosine on BIST B+
December 5, 2022
Ionic Modifier Effect on Selectivity of Separation of Deoxyguanosine, Guanine and Guanosine on BIST B+ by SIELC Technologies.
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analyses of Deoxyguanosine, Guanine and Guanosine
Condition
Column | BIST B+, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN |
Buffer | H3PO4, H2SO4, HFGA (Hexafluoroglutaric acid) – 0.2%, |
Flow Rate | 1.0 ml/min |
Detection | UV 260 nm |
Peak Retention Time |
Description
Class of Compounds | Nucleosides |
Analyzing Compounds | Deoxyguanosine, Guanine and Guanosine |
Application Column
BIST B+
BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.
Select optionsGuanine
Guanosine
HPLC Method for Analysis of Deoxyguanosine, Guanine and Guanosine on BIST B+
December 5, 2022
HPLC Method for Analysis of Deoxyguanosine, Guanine and Guanosine on BIST B+ by SIELC Technologies.
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analyses of Deoxyguanosine, Guanine and Guanosine
Condition
Column | BIST B+, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN – 85% |
Buffer | H3PO4 – 0.2% |
Flow Rate | 1.0 ml/min |
Detection | UV 260 nm |
Peak Retention Time | 9.6, 11.2, 12.8 min |
Description
Class of Compounds | Nucleosides |
Analyzing Compounds | Deoxyguanosine, Guanine and Guanosine |
Application Column
BIST B+
BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.
Select optionsGuanine
Guanosine
HPLC Separation of Guanosine, Deoxyguanosine, Adenosine, Deoxyadenosine on Newcrom AH Column
May 25, 2021
Separation type: Liquid Chromatography Mixed-mode
Nucleosides are the building blocks for DNA and RNA as well as other roles in biomechanical processes such as signal transduction. By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, nucleosides: guanosine, deoxyguanosine, adenosine, and deoxyadenosine, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and H3PO4 as a buffer. UV detection at 210 nm.
Column | Newcrom AH, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 20/80% |
Buffer | H3PO4 – 0.5% |
Flow Rate | 1.0 ml/min |
Detection | UV, 210 nm |
Class of Compounds |
Nucleoside, Hydrophilic, Ionizable |
Analyzing Compounds | Guanosine, Deoxyguanosine, Adenosine, Deoxyadenosine |
Application Column
Newcrom AH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsDeoxyadenosine
Deoxyguanosine
Guanosine
HPLC Separation of Nucleosides and Deoxynucleosides
July 23, 2012
Column | Sharc 1, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN/MeOH |
Buffer | AmFm, Formic acid |
Flow Rate | 1.0 ml/min |
Detection | UV, 270 nm |
Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements |
Analyzing Compounds | Thymidine, Uridine, Deoxyadenosine, Adenosine, Deoxyguanosine, Guanosine, Deoxycytidine, Cytidine |
Application Column
SHARC 1
The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.
Select optionsCytidine
Deoxyadenosine
Deoxycytidine
Deoxyguanosine
Guanosine
Thymidine
Uridine
HPLC Separation of Thymidine, Uridine, Adenosine, Guanosine, and Cytidine Using the Hydrogen Bonding Method
June 15, 2012
Application Notes: Nucleosides are glycosylamines consisting of nucleobase linked to ribose or deoxyribose sugar and are building blocks for DNA and RNA. These compounds are very polar and contain groups available for hydrogen bonding interaction. Thymidine, uridine, adenosine, guanosine and cytidine were separated using a hydrogen-bonding method. There is a strong correlation between the retention time and mobile phase composition. The strength of hydrogen-bonding interaction increases as the number of hydroxyls in the analytes increase. Additionally the order of elution for compounds depends on the ratio of the mobile phases: acetonitrile and methanol. Our method is compatible with LC/MS and preparative chromatography.
Application Columns: SHARC 1, 3.2×100 mm, 5 um, 100A, To learn more about SHARC 1 columns click here. To order this column click here. To see more chromatographic separations check our web site.
Application Compounds: Thymidine, uridine, adenosine, guanosine and cytidine
Column | Sharc 1, 3.2×100 mm, 5 µm, 100A |
Mobile Phase | MeCN/MeOH |
Buffer | AmFm, Formic acid |
Flow Rate | 1.0 ml/min |
Detection | UV, 270 nm |
Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements |
Analyzing Compounds | Thymidine, Uridine, Adenosine, Guanosine, Cytidine |
Application Column
SHARC 1
The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.
Select optionsCytidine
Guanosine
Thymidine
Uridine
HPLC Separation of Nucleic Bases at pH 4 and 5 on Obelisc N
March 3, 2007
Nucleic bases are biological compounds found in genetic molecules (DNA, RNA). They can be separated on an Obelisc N column, which offers very polar characteristics and can be used with positively or negatively charged groups. Closely-eluted adenosine and uridine can be further separated by simply adjusting the pH of the mobile phase. Mobile phase is water and acetonitrile (MeCN, ACN) with Ammonium Acetate as buffer. UV detection at 250nm.
Column | Obelisc N, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN -90% |
Buffer | AmAc |
Flow Rate | 1.0 ml/min |
Detection | UV, 250 nm |
Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements |
Analyzing Compounds | Uracil, Uridine, Adenosine, Guanosine, Cytidine, Cytosine |
Application Column
Obelisc N
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsCytidine
Cytosine
Guanosine
Uracil
Uridine