Ionic Modifier Effect on Selectivity of Separation of Deoxyguanosine, Guanine and Guanosine on BIST B+ by SIELC Technologies.
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analyses of Deoxyguanosine, Guanine and Guanosine
Condition
Column | BIST B+, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN |
Buffer | H3PO4, H2SO4, HFGA (Hexafluoroglutaric acid) – 0.2%, |
Flow Rate | 1.0 ml/min |
Detection | UV 260 nm |
Peak Retention Time |
Description
Class of Compounds | Nucleosides |
Analyzing Compounds | Deoxyguanosine, Guanine and Guanosine |
Application Column
BIST B+
BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.
Select optionsGuanine
Guanosine