| CAS Number | 73-03-0 |
|---|---|
| Molecular Formula | C10H13N5O3 |
| Molecular Weight | 251.247 |
| InChI Key | OFEZSBMBBKLLBJ-BAJZRUMYSA-N |
| LogP | -0.728 |
| Synonyms |
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Applications:
HPLC Method for Analysis of 3′-Deoxyadenosine and 2′-Deoxyadenosine on BIST B+ Column
December 1, 2022
HPLC Method for Cordycepin, Deoxyadenosine on BIST B+ by SIELC Technologies
Cordycepin is an adenosine analogue with the chemical formula C10H13N5O3. It is reported to prevent cell reproduction in various cancer cells. It also might possess antioxidant and anti-inflammatory properties, when considered in addition with it’s ability to cross the blood-brain barrier, it may become widely used in pharmaceuticals.
Deoxyadenosine is a deoxyribonucleoside with the chemical formula C10H13N5O3. It is a derivative of adenosine. High presence of it can kill T lymphocytes and kill those cells, leading to adenosine deaminase severe combined immunodeficiency disease, also known as ADA-SCID.
Cordycepin, Deoxyadenosine can be retained and analyzed using the BIST B+ stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Phosphoric Acid buffer. Detection is performed using UV.
Condition
| Column | BIST B+, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 85% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260 nm |
| Peak Retention Time | 8.2, 9.8 min |
Description
| Class of Compounds | Nucleosides |
| Analyzing Compounds | Cordycepin, Deoxyadenosine |
Application Column
BIST B+
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Deoxyadenosine
HPLC Separation of Adenosine, Cordycepin and Adenine on Newcrom AH Column
April 8, 2020
HPLC Method for Adenosine, Cordycepin, Adenine on Newcrom AH by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Adenosine, Cordycepin, Adenine.
Adenosine is a key building block of energy-carrying molecules with the chemical formula C10H13N5O4. It has a variety of other uses, including being a inhibitory neurotransmitter which helps with sleep and acting as a blood flow regulator. Medicinally, it is used as treatment for supraventricular tachycardia (SVT). You can find detailed UV spectra of Adenosine and information about its various lambda maxima by visiting the following link.
Cordycepin is an adenosine analogue with the chemical formula C10H13N5O3. It is reported to prevent cell reproduction in various cancer cells. It also might possess antioxidant and anti-inflammatory properties, when considered in addition with it’s ability to cross the blood-brain barrier, it may become widely used in pharmaceuticals.
Due to cordycepin having a very similar structure to adenosine, it has shown to have inhibitive properties on the COVID-19 coronavirus. However, due to their similar structures, the separation of the two sugars can be challenging. Both sugars can be separated isocratically in about six minutes on the Newcrom AH mixed-mode column, which has both hydrophobic and cationic exchange properties. The mobile phase consists of acetonitrile (ACN, MeCN) and water with ammonium formate as a buffer which makes it mass-spec (MS) compatible. It can also be UV detected at 260nm.
| Column | Newcrom AH, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | AmFm pH 3.0- 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260 nm, MS-compatible mobile phase |
| Class of Compounds | Hydrophilic, Drug, Xanthine, Nucleobase |
| Analyzing Compounds | Adenosine, Cordycepin, Adenine |
Application Column
Newcrom AH
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Adenosine
Cordycepin
Separation of Cordycepin on Newcrom R1 HPLC column
February 16, 2018
Cordycepin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options
